Description

Azido-PEG3-NHS Ester is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysuccinimide (NHS) ester and a terminal azide group, connected by a triethylene glycol spacer. This compound is essential for creating stable, site-specific conjugations in bioconjugation workflows, particularly for click chemistry applications. It is primarily used by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostics industries for labeling proteins, antibodies, and other biomolecules.

Application

• Bioconjugation and Protein Labeling: Conjugation of azide-modified biomolecules (via the NHS ester) to alkyne-bearing probes, surfaces, or other molecules via copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Antibody-Drug Conjugate (ADC) Development: Site-specific attachment of cytotoxic payloads or fluorescent tags to antibodies for targeted therapeutics and imaging.
• Surface Functionalization: Modification of nanoparticles, microarrays, or solid supports with azide groups for subsequent bio-orthogonal coupling.
• Polymer Chemistry: Synthesis of functionalized PEG-based polymers and hydrogels with clickable handles.
• Diagnostic Assay Development: Creating detection complexes and immunoassay components with enhanced stability and specificity.
• Chemical Biology Probes: Introducing azide tags into cellular systems for metabolic labeling and pull-down experiments.

Basic Information

Product Name	Azido-PEG3-NHS Ester
CAS No.	1245718-89-1
Molecular Formula	C17H26N4O8
Molecular Weight	414.41 g/mol
Synonyms	NHS-PEG3-Azide; Azido-PEG3-NHS; 3-Azidopropyl-PEG3-NHS ester; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-(3-azidopropoxy)ethoxy]ethoxy]propanoate; N-(3-Azidopropyl)-3,6,9-trioxaundecanedioic acid imide; PEG3 Azide NHS Ester; Azido-tri(ethylene glycol)-NHS ester
EINECS	Contact for details

Quality Control

Our Azido-PEG3-NHS Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for critical research and production applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional group integrity. We adhere to relevant quality management standards to support our customers in GMP and ISO-compliant environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent hydrolysis of the NHS ester moiety. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or oil
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Assay (NHS Ester)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.