Description

4-Azido-D-Phenylalanine Hcl is a specialized, non-canonical amino acid derivative designed for advanced biochemical research and development. This compound is a critical building block for site-specific protein labeling and bioorthogonal chemistry, enabling precise manipulation and study of biological systems. It is primarily utilized by researchers and manufacturers in the fields of chemical biology, proteomics, and pharmaceutical development for creating novel bioconjugates and therapeutic agents.

Application

• Bioorthogonal Labeling: A key reagent for strain-promoted azide-alkyne cycloaddition (SPAAC) and copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry reactions for site-specific protein modification.
• Chemical Biology Research: Used to incorporate azide functional groups into proteins and peptides for subsequent conjugation with fluorescent dyes, affinity tags, or other biomolecules.
• Proteomics and Biomarker Discovery: Enables the tagging and enrichment of newly synthesized proteins for mass spectrometry analysis and functional studies.
• Antibody-Drug Conjugate (ADC) Development: Serves as a linker component for constructing stable, homogeneous ADCs with precise drug-to-antibody ratios.
• Materials Science: Facilitates the surface functionalization of nanomaterials and polymers for biomedical and diagnostic applications.
• Enzyme and Receptor Studies: Used to create activity-based probes to investigate enzyme mechanisms and receptor-ligand interactions.

Basic Information

Product Name	4-Azido-D-Phenylalanine Hcl
CAS No.	1241681-80-0
Molecular Formula	C9H10N4O2 • HCl
Molecular Weight	242.66 g/mol (Free base: 206.20)
Synonyms	D-4-Azidophenylalanine hydrochloride; (R)-2-Amino-3-(4-azidophenyl)propanoic acid hydrochloride; p-Azido-D-Phe-OH • HCl; 4-N3-D-Phe-OH • HCl; Fmoc-4-Azido-D-Phenylalanine; Azido-D-Phenylalanine (HCl salt); Non-canonical amino acid derivative; Bioorthogonal amino acid
EINECS	Contact for details

Quality Control

Our 4-Azido-D-Phenylalanine Hcl is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and chiral analysis to ensure enantiomeric integrity. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting compliance with our stringent in-house specifications. We support research requiring traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This compound is light-sensitive (store away from light) and hygroscopic. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture absorption. For optimal performance, use immediately after preparation of solutions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95.0%
Chiral Purity (HPLC)	≥ 98.0% ee (D-isomer)
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.