Description

Cy5-Alkyne is a near-infrared fluorescent labeling reagent featuring a terminal alkyne group for bioorthogonal click chemistry conjugation. This compound is essential for researchers and developers requiring high-sensitivity, low-background labeling of biomolecules such as proteins, nucleic acids, and glycans. It is primarily used in the life sciences, pharmaceutical R&D, and diagnostics sectors for advanced applications including super-resolution microscopy, in vivo imaging, and multiplexed assays. Cy5-Alkyne CAS NO 1223357-57-0 offers a powerful tool for creating custom fluorescent probes with excellent photostability and deep tissue penetration.

Application

• Bioorthogonal Labeling: Site-specific fluorescent tagging of azide-modified biomolecules via copper-catalyzed or copper-free click chemistry reactions.
• Live-Cell Imaging: Real-time visualization and tracking of cellular processes, receptor trafficking, and protein dynamics in the near-infrared window.
• Proteomics & Biomarker Discovery: Conjugation to antibodies, peptides, or other targeting agents for use in fluorescence-activated cell sorting (FACS), western blotting, and ELISA development.
• Nucleic Acid Labeling: Preparation of fluorescent DNA or RNA probes for fluorescence in situ hybridization (FISH), PCR, and next-generation sequencing applications.
• Material Science Functionalization: Incorporation into polymers, nanoparticles, or surfaces to create fluorescent materials for sensing and imaging.
• In Vivo Imaging Agent Synthesis: Key building block for developing targeted near-infrared fluorescent probes for preclinical animal imaging studies.

Basic Information

Product Name	Cy5-Alkyne
CAS No.	1223357-57-0
Molecular Formula	C37H45N3O2+
Molecular Weight	563.78 g/mol
Synonyms	Cy5 Alkyne; Sulfo-Cyanine5 Alkyne; Cyanine5 Alkyne; 1-(5-Carboxypentyl)-2-((1E,3E)-5-((E)-1-(5-(hex-5-yn-1-yloxy)-1,3-dioxo-1,3-dihydro-2H-benzo[de]isoquinolin-2-yl)ethylidene)-4-methylpenta-1,3-dien-1-yl)-3,3-dimethyl-3H-indol-1-ium; 2-[5-[2-[5-[2-[2-(5-Hexyn-1-yloxy)-1,3-dioxo-1,3-dihydro-2H-benzo[de]isoquinolin-2-ylidene]ethylidene]-4-methyl-3-oxo-1-cyclopenten-1-yl]ethenyl]-3,3-dimethyl-1-pentyl-3H-indolium-5-carboxylate
EINECS	Contact for details

Quality Control

Our Cy5-Alkyne is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure high batch-to-batch consistency and performance reliability. A comprehensive Certificate of Analysis (COA) detailing identity, purity, and spectral data is provided with every shipment to support your research and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive). Allow the vial to warm to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark blue to green solid or film
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with reference structure
Purity (HPLC)	≥ 95% (Area % at 650 nm)
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in DMSO, DMF, methanol
Extinction Coefficient (ε)	≥ 250,000 L·mol⁻¹·cm⁻¹ (in methanol)
Fluorescence Emission (λmax)	~670 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.