Description

Adenosine, n-Acetyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-(Trimethylsilyl)-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite] is a highly specialized, protected phosphoramidite monomer used in the automated solid-phase synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the production of DNA and RNA strands for research and therapeutic applications. It is an essential raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on nucleic acid synthesis.

Application

• Oligonucleotide Synthesis: As a key building block (phosphoramidite) in automated solid-phase DNA/RNA synthesizers.
• Therapeutic Nucleic Acid Development: For the research-scale and GMP production of antisense oligonucleotides, siRNA, and aptamers.
• Diagnostic Probe Manufacturing: Used to synthesize labeled probes for PCR, microarrays, and next-generation sequencing (NGS).
• Academic & Industrial Research: Enables the custom synthesis of modified oligonucleotides for structural, biochemical, and molecular biology studies.
• Synthesis of Modified Nucleosides: Serves as an intermediate for introducing specific modifications at the adenosine position in oligonucleotide sequences.

Basic Information

Item	Details
Product Name	Adenosine, n-Acetyl-5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-o-(Trimethylsilyl)-, 3'-[2-Cyanoethyl N,n-Bis(1-Methylethyl)Phosphoramidite]
CAS No.	1220889-84-8
Molecular Formula	C48H63N6O8PSi
Molecular Weight	887.11 g/mol
Synonyms	5'-O-DMT-2'-O-TMS-N-acetyl-adenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; DMT-rA(Ac)-2'-TMS-3'-CE Phosphoramidite; N-Acetyl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(trimethylsilyl)adenosine-3'-[O-(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; Ac-rA(DMT-2'TMS) Phosphoramidite; Protected Adenosine Phosphoramidite; RNA Adenosine Phosphoramidite (2'-O-TMS protected); 2'-O-TMS-Adenosine Phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of our phosphoramidite monomers is produced and tested under strict quality management systems. We guarantee high purity and reliable performance for critical oligonucleotide synthesis. Quality is verified by advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to ensure conformity with specifications. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and functional performance is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed container to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Solubility	Clear, colorless solution in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.