Description

Rac cis-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester is a high-purity chemical reference standard and synthetic intermediate of significant importance in analytical and pharmaceutical research. This compound is critical for the accurate quantification and metabolic profiling of nicotine and its derivatives, ensuring data integrity in clinical and forensic studies. It is primarily utilized by research institutions, analytical laboratories, and pharmaceutical companies engaged in biomarker analysis, drug metabolism studies, and the development of smoking cessation therapies.

Application

• Analytical Reference Standard: Primary use as a certified standard for the precise calibration and validation of HPLC, LC-MS, and GC-MS methods in bioanalytical laboratories.
• Metabolite Research: Essential for studying the metabolic pathways and pharmacokinetics of nicotine and cotinine in clinical and preclinical research.
• Biomarker Development: Used in the development and verification of diagnostic assays to measure tobacco smoke exposure and related health studies.
• Pharmaceutical Intermediate: Serves as a key synthetic building block in the research and development of novel pharmaceutical agents targeting nicotine addiction.
• Forensic Toxicology: Employed as a qualitative and quantitative standard in forensic labs for the accurate analysis of nicotine metabolites in biological samples.
• Quality Control (QC) Material: Used as a system suitability test compound and an impurity marker in the quality control of related pharmaceutical products.

Basic Information

Product Name	Rac cis-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester
CAS No.	1217778-14-7
Molecular Formula	C12H14N2O4
Molecular Weight	250.25 g/mol
Synonyms	3'-Hydroxycotinine methyl ester; Methyl 3'-hydroxycotinine-3-carboxylate; trans-3'-Hydroxycotinine methyl ester (racemic mixture); Nicotine metabolite derivative; Cotinine carboxylic acid methyl ester, hydroxylated; (±)-cis-3'-Hydroxycotinine-3-carboxylic acid methyl ester; 1-Methyl-5-(3-pyridyl)-2-pyrrolidinone-3-carboxylic acid, 3'-hydroxy-, methyl ester
EINECS	Contact for details

Quality Control

Our Rac cis-3'-Hydroxy Cotinine-3-Carboxylic Acid Methyl Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the high standards required for research and analytical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical work.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. For long-term storage, consider storing at 2-8°C to ensure optimal stability.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Purity (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 0.5%
Residue on Ignition	≤ 0.1%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.