Description

3',5'-Bis-o-[(1,1-Dimethylethyl)Dimethylsilyl]-α-[[(1,1-Dimethylethyl)Dimethylsilyl]Oxy]Thymidine is a sophisticated, multi-silyl protected derivative of thymidine, a key nucleoside building block. This compound is critically important for enabling complex oligonucleotide synthesis by providing robust protection for reactive hydroxyl groups, preventing unwanted side reactions. It is primarily needed by research institutions and pharmaceutical companies engaged in the development of novel therapeutics, including antisense oligonucleotides, siRNA, and other nucleic acid-based drugs.

Application

• Oligonucleotide Synthesis: Serves as a critical protected intermediate in the solid-phase and solution-phase synthesis of DNA and RNA sequences.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides designed to target specific mRNA sequences.
• siRNA and miRNA Synthesis: Acts as a building block for the manufacture of small interfering RNA (siRNA) and microRNA (miRNA) for gene silencing applications.
• Pharmaceutical Intermediates: A key starting material or intermediate in the multi-step synthesis of nucleoside-based active pharmaceutical ingredients (APIs).
• Chemical Biology Research: Employed in academic and industrial labs for studying nucleic acid structure, function, and for probe development.
• Process Development: Used in scaling up and optimizing synthetic routes for nucleotide analogs under controlled, protected conditions.

Basic Information

Product Name	3',5'-Bis-o-[(1,1-Dimethylethyl)Dimethylsilyl]-α-[[(1,1-Dimethylethyl)Dimethylsilyl]Oxy]Thymidine
CAS No.	1210427-80-7
Molecular Formula	C31H62N2O6Si3
Molecular Weight	659.15 g/mol
Synonyms	3',5'-Bis-O-(tert-butyldimethylsilyl)-2'-O-(tert-butyldimethylsilyl)thymidine; 3',5'-Di-O-TBDMS-2'-O-TBDMS-thymidine; TBDMS-protected Thymidine; 1-[(2R,3S,5R)-3-[bis[(1,1-dimethylethyl)dimethylsilyl]oxy]-5-[[(1,1-dimethylethyl)dimethylsilyl]oxymethyl]oxolan-2-yl]-5-methyl-pyrimidine-2,4-dione; Fully Silylated Thymidine Derivative
EINECS	Contact for details

Quality Control

Our 3',5'-Bis-o-[(1,1-Dimethylethyl)Dimethylsilyl]-α-[[(1,1-Dimethylethyl)Dimethylsilyl]Oxy]Thymidine is produced and handled under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the exacting standards required for pharmaceutical research and development. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and must be kept under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment to maintain stability and purity. Avoid prolonged exposure to air and moisture.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.