Description

Azido-PEG8-NHS Ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide group and an N-hydroxysuccinimide (NHS) ester. This compound is essential for creating stable, covalent linkages between biomolecules via click chemistry and amine-reactive conjugation. It is a critical reagent for researchers and manufacturers in bioconjugation, pharmaceutical development, and advanced material science, enabling the precise modification of proteins, peptides, surfaces, and nanoparticles.

Application

• Bioconjugation and Protein Labeling: Site-specific modification of antibodies, enzymes, and other proteins via NHS-ester coupling to lysine residues, followed by azide-alkyne cycloaddition (CuAAC or SPAAC).
• ADC (Antibody-Drug Conjugate) Development: Construction of linker-payload complexes for targeted cancer therapies, utilizing the PEG spacer to improve solubility and pharmacokinetics.
• Surface Functionalization: Immobilization of biomolecules onto chips, beads, or nanoparticles for biosensor development, diagnostic assays, and microarray platforms.
• Polymer and Biomaterial Synthesis: Incorporation into hydrogels, drug delivery systems, and smart materials to introduce bio-orthogonal handles for further modification.
• Chemical Biology Probes: Synthesis of activity-based probes and imaging agents for studying biological processes in vitro and in vivo.
• Peptide and Oligonucleotide Modification: Conjugation of tags, fluorophores, or other functional groups to peptides and oligonucleotides.

Basic Information

Product Name	Azido-PEG8-NHS Ester
CAS No.	1204834-00-3
Molecular Formula	C29H50N4O13
Molecular Weight	662.73 g/mol
Synonyms	NHS-PEG8-Azide; Azido-PEG8-NHS Ester; N-(Azido-PEG8)-N-hydroxysuccinimide ester; α-Azido-ω-NHS-PEG8; 8-Azido-3,6,9,12,15,18,21,24-octaoxahexacosanoic acid 2,5-dioxo-1-pyrrolidinyl ester; PEG8 Azide NHS Ester; Azido-PEG8-OSu
EINECS	Contact for details

Quality Control

Our Azido-PEG8-NHS Ester is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency for sensitive research and development applications. Each lot is characterized by NMR, HPLC, and mass spectrometry to confirm identity, purity, and functional group integrity. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term stability, store under inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90%
Functional Group Assay (NHS Ester)	≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.