Description

n-(2-(2-Azidoethoxy)Ethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide is a specialized biotin derivative, engineered for advanced bioconjugation and labeling applications. Its molecular design, featuring a reactive azido group tethered to the biotin core, makes it an essential tool for click chemistry and affinity-based purification systems. This compound is primarily utilized by researchers and manufacturers in the fields of proteomics, drug discovery, and diagnostic assay development, where precise biomolecule tagging is required.

Application

• Click Chemistry Reagent: Acts as a key intermediate for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions to label biomolecules.
• Affinity Chromatography Ligand: Used to functionalize solid supports (e.g., agarose beads) for the purification of streptavidin/avidin-tagged proteins.
• Proteomic Research: Enables site-specific biotinylation of proteins and peptides for pull-down assays and mass spectrometry analysis.
• Diagnostic Kit Development: Incorporated into the design of ELISA and lateral flow assays for high-sensitivity detection.
• Drug Delivery Systems: Serves as a linker for creating targeted biotinylated drug conjugates.
• Cell Surface Engineering: Facilitates the labeling and tracking of cell surface receptors in flow cytometry and imaging.
• Material Science: Used to create biotin-functionalized surfaces for biosensor and microarray fabrication.

Basic Information

Product Name	n-(2-(2-Azidoethoxy)Ethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide
CAS No.	1204085-48-2
Molecular Formula	C16H25N7O3S
Molecular Weight	395.48 g/mol
Synonyms	Biotin-PEG3-Azide; Biotin-PEG3-N3; 5-((3aR,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)-N-(2-(2-(2-azidoethoxy)ethoxy)ethyl)pentanamide; Biotin Azide; Biotin-PEG-Azide; Biotin-triethyleneglycol-azide; Azido-PEG3-Biotin; N-(2-(2-(2-Azidoethoxy)ethoxy)ethyl)-5-((3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamide
EINECS	Contact for details

Quality Control

Our production of n-(2-(2-Azidoethoxy)Ethyl)-5-((3As,4S,6Ar)-2-Oxohexahydro-1H-Thieno[3,4-D]Imidazol-4-Yl)Pentanamide adheres to stringent quality protocols suitable for research and diagnostic applications. Each batch undergoes comprehensive analytical testing, including HPLC, NMR, and mass spectrometry, to verify identity, purity, and stability. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring full traceability and compliance with your project specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. The product is moisture-sensitive; keep the original container sealed in a dry environment. Allow the vial to equilibrate to room temperature before opening to minimize condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.