Description

Calcein Tetraethyl Ester is a cell-permeant, non-fluorescent ester derivative of the calcium-sensitive fluorophore Calcein. This compound is critically important as a vital stain and viability indicator in cell biology and live-cell imaging applications. Researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences industries rely on it for assessing cell membrane integrity, cytotoxicity, and apoptosis.

Application

• Live/Dead Cell Viability Assays: Used as a vital stain to distinguish between live cells with intact esterase activity and compromised dead cells.
• Cytotoxicity Screening: Essential for high-throughput screening (HTS) of drug candidates and chemical libraries for toxic effects.
• Apoptosis Detection: Employed in fluorescence microscopy and flow cytometry to monitor programmed cell death.
• Cell Membrane Integrity Studies: Acts as a fluorescent marker for cells with intact plasma membranes following experimental treatments.
• Neuroscience Research: Utilized in neuronal cell culture studies to assess health and viability.
• Microbiology Applications: Can be applied in certain bacterial viability assays.

Basic Information

Product Name	Calcein Tetraethyl Ester
CAS No.	1170856-93-5
Molecular Formula	C46H50N2O23
Molecular Weight	995.89 g/mol
Synonyms	Calcein-AM; Calcein Acetoxymethyl Ester; Calcein AM; 3,3′-Bis[N,N-bis(carboxymethyl)aminomethyl]fluorescein Tetraacetoxymethyl Ester; 3,3′-Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein Tetraacetoxymethyl Ester; 3,3′-Bis[N,N-di(carboxymethyl)aminomethyl]fluorescein Tetra-AM Ester; Fluorexon Tetraacetoxymethyl Ester; Cell Viability Stain
EINECS	Contact for details

Quality Control

Our Calcein Tetraethyl Ester is produced and tested to meet the stringent requirements of research and diagnostic applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure consistent performance and reliability in sensitive biological assays. A comprehensive Certificate of Analysis (COA) detailing purity, fluorescence properties, and absence of critical impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C. Under these conditions, the product is stable for at least 24 months. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange-yellow solid or film
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Fluorescence Properties	Ex/Em ~495 nm/~515 nm in aqueous buffer (post-esterase cleavage)
Solubility	Soluble in DMSO, DMF; sparingly soluble in aqueous buffer
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.