Description

Cyanine3 Azide CAS NO 1167421-28-4 is a near-infrared fluorescent dye functionalized with a reactive azide group, enabling bioorthogonal click chemistry for labeling biomolecules. This compound is critical for researchers requiring high-sensitivity detection and stable covalent conjugation in complex biological systems without interfering with native biochemical processes. It is essential for professionals in pharmaceutical R&D, biotechnology, and academic research focused on bioimaging, diagnostics, and proteomic analysis.

Application

• Bioorthogonal Labeling: Covalent tagging of proteins, antibodies, and oligonucleotides via copper-free click chemistry with alkynes.
• Live-Cell Imaging: Real-time visualization and tracking of cellular processes and biomolecule localization with minimal background fluorescence.
• Proteomics & Biomarker Discovery: Fluorescent labeling of peptides and proteins for gel-based analysis, western blotting, and mass spectrometry.
• Diagnostic Assay Development: Conjugation to detection probes for use in ELISA, lateral flow assays, and in vitro diagnostics (IVD).
• Flow Cytometry: Staining of cell surface markers and intracellular targets for high-throughput cell analysis and sorting.
• Nanoparticle & Drug Conjugate Tracking: Fluorescent tagging of drug delivery vehicles, liposomes, and polymeric nanoparticles to monitor biodistribution.
• In Vivo Imaging: Preclinical research applications for non-invasive optical imaging in small animal models.
• Materials Science: Surface functionalization and labeling of polymers and biomaterials for research and development.

Basic Information

Product Name	Cyanine3 Azide
CAS No.	1167421-28-4
Molecular Formula	C34H40ClN6O+
Molecular Weight	583.18 g/mol
Synonyms	Cy3 Azide; Sulfo-Cyanine3 azide; Cy3 N3; 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((E)-3-((E)-1-(6-azidohexyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium chloride; Cyanine 3 azide; Azide-Cy3; Indocyanine green azide derivative; NIR dye azide
EINECS	Contact for details

Quality Control

Our Cyanine3 Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry for identity verification, to ensure compliance with research-grade specifications. Certificates of Analysis (COA) detailing lot-specific results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. Under these conditions, the product is stable for at least 24 months from the date of receipt. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]+
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, methanol
Extinction Coefficient	~150,000 cm-1M-1 at λmax
Fluorescence Emission	~570 nm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.