Description

5(6)-Carboxy-Hexachlorofluorescein is a chemically modified derivative of fluorescein, featuring multiple chlorine atoms and a carboxylic acid functional group. This modification is critical for enhancing its photostability and altering its spectral properties, making it a highly valuable intermediate and active component in advanced applications. It is primarily sought by manufacturers and researchers in the life sciences, analytical chemistry, and specialty materials sectors who require precise, stable fluorescent markers and probes.

Application

• Fluorescent Probe Synthesis: A key intermediate for creating custom fluorescent probes and tags used in bioimaging and diagnostic assays.
• Bioconjugation: The carboxylic acid group enables covalent attachment to antibodies, proteins, and oligonucleotides for fluorescence-activated cell sorting (FACS) and immunohistochemistry.
• Analytical Reagents: Used in the formulation of high-sensitivity detection kits for enzymatic activity and molecular biology applications.
• Sensor Development: Serves as a core fluorophore in the design of chemical sensors and pH indicators due to its tunable emission properties.
• Material Science: Incorporated into polymers and nanoparticles to create fluorescent materials for security inks or tracking applications.
• Research & Development: A vital tool in academic and industrial R&D for studying molecular interactions and developing new fluorescent methodologies.

Basic Information

Product Name	5(6)-Carboxy-Hexachlorofluorescein
CAS No.	1166843-68-0
Molecular Formula	C21H8Cl6O7
Molecular Weight	616.01 g/mol
Synonyms	5(6)-Carboxy-2',4',5',7'-tetrachloro-6-hydroxy-3H-xanthen-3-one; Hexachlorofluorescein carboxylic acid; 5(6)-Carboxy-2',4',5',7'-tetrachlorofluorescein; 6-Hydroxy-3-oxo-3H-xanthene-9-carboxylic acid, 2',4',5',7'-tetrachloro-5(6)-carboxy derivative; 5(6)-COOH-HCF
EINECS	Contact for details

Quality Control

Our production of 5(6)-Carboxy-Hexachlorofluorescein adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity, essential for sensitive research and manufacturing processes. Each lot is analyzed using advanced techniques like HPLC and NMR to verify identity and quantify impurities. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container must be kept tightly sealed in a desiccated environment to prevent degradation. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Heavy Metals	≤ 20 ppm
Residual Solvents	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.