Description

Fluorescein-Peg2-Azide is a bifunctional fluorescent labeling reagent designed for bioorthogonal conjugation via click chemistry. This compound matters for researchers and manufacturers who require precise, covalent attachment of fluorescent tags to biomolecules under mild, aqueous conditions. It is primarily needed by professionals in bioconjugation, pharmaceutical R&D, and diagnostic assay development for creating fluorescent probes, antibody-drug conjugates (ADCs), and cellular imaging agents.

Application

• Bioconjugation and Probe Synthesis: Used for site-specific fluorescent labeling of proteins, antibodies, peptides, and oligonucleotides via copper-free or copper-catalyzed azide-alkyne cycloaddition (CuAAC).
• Antibody-Drug Conjugate (ADC) Development: Serves as a model fluorescent payload or linker-payload component in the research and development of targeted therapeutics.
• Cellular and In Vivo Imaging: Enables the visualization and tracking of azide-modified biomolecules, glycans, or metabolites within live cells or tissues.
• Diagnostic Assay Development: Utilized in the creation of fluorescent sensors, lateral flow assays, and ELISA-based detection systems requiring high-sensitivity signal generation.
• Materials Science and Surface Functionalization: Applied to introduce fluorescent properties to polymers, nanoparticles, or solid surfaces for analytical or sensing applications.
• Chemical Biology Research: A key tool for studying biological processes, protein interactions, and metabolic pathways through fluorescence-based detection methods.

Basic Information

Product Name	Fluorescein-Peg2-Azide
CAS No.	1146195-72-3
Molecular Formula	C33H33N5O9
Molecular Weight	643.65 g/mol
Synonyms	5-FAM-PEG2-Azide; 5-Carboxyfluorescein-PEG2-Azide; 6-FAM-PEG2-N3; Fluorescein-5-PEG2-Azide; 5(6)-Carboxyfluorescein-PEG2-Azide; Azido-PEG2-5-FAM; N3-PEG2-FAM; 5-Isomer FAM-PEG2-Azide
EINECS	Contact for details

Quality Control

Our Fluorescein-Peg2-Azide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and development applications. Certificates of Analysis (COA) detailing lot-specific results are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Orange to red solid
Identification (HPLC/MS)	Conforms
Purity (HPLC)	≥95%
Water Content (KF)	≤1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.