Description

Cy5.5-Cooh is a near-infrared (NIR) fluorescent dye derivative featuring a carboxylic acid functional group, enabling covalent conjugation to biomolecules. This compound is essential for creating highly sensitive and stable fluorescent probes for deep-tissue imaging and multiplexed detection. It is primarily utilized by researchers and manufacturers in the biotechnology, pharmaceutical, and in vitro diagnostics (IVD) sectors for advanced bioanalytical applications.

Application

• Bioconjugation and Probe Development: Covalent labeling of antibodies, peptides, proteins, and oligonucleotides via its carboxylic acid group for NIR fluorescence applications.
• In Vivo Imaging: Synthesis of contrast agents for non-invasive deep-tissue fluorescence imaging in preclinical animal studies, leveraging its NIR emission to minimize tissue autofluorescence.
• Diagnostic Assays: Manufacturing of fluorescent reagents for high-sensitivity immunoassays, flow cytometry, and fluorescence microscopy.
• Drug Delivery & Theranostics: Conjugation to drug carriers or nanoparticles for tracking biodistribution and developing combined therapeutic and diagnostic (theranostic) platforms.
• Biomarker Detection: Development of sensitive probes for the detection and quantification of specific cellular targets or biomarkers in complex biological matrices.

Basic Information

Product Name	Cy5.5-Cooh
CAS No.	1144107-80-1
Molecular Formula	C44H49N3O6S2+
Molecular Weight	788.00 g/mol (for cation)
Synonyms	Cy5.5 carboxylic acid; Sulfo-Cyanine5.5 carboxylic acid; Cyanine5.5-COOH; 1H-Benz[e]indolium, 2-[5-[1,1-dimethyl-3-(4-sulfobutyl)-1,3-dihydro-2H-benz[e]indol-2-ylidene]-1,3-pentadien-1-yl]-1,1-dimethyl-3-(4-carboxybutyl)-, inner salt; NIR dye carboxylic acid; Cy5.5 NHS ester precursor; Near-infrared fluorescent dye acid
EINECS	Contact for details

Quality Control

Our Cy5.5-Cooh is manufactured under strict quality protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and NMR for structural confirmation. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	680 - 690 nm
Emission Maximum (λem)	705 - 715 nm
Extinction Coefficient (ε)	≥ 190,000 cm-1M-1
Water Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.