Description

Cy3.5-Cooh CAS NO 1144107-79-8 is a carboxylic acid-functionalized cyanine dye derivative, a crucial intermediate for creating stable, fluorescent bioconjugates. Its primary value lies in providing a reactive handle for covalent attachment to biomolecules like proteins, peptides, and oligonucleotides, enabling sensitive detection and imaging. This product is essential for researchers and manufacturers in the life sciences, diagnostic assay development, and pharmaceutical research sectors who require reliable, high-purity fluorescent labeling reagents.

Application

• Bioconjugation and Labeling: Covalent attachment to antibodies, proteins, and peptides for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and Western blotting.
• Nucleic Acid Labeling: Fluorescent tagging of oligonucleotides, DNA, and RNA probes for in situ hybridization (FISH) and real-time PCR applications.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent lateral flow assays, microarrays, and other clinical diagnostic tools.
• Bioimaging and Microscopy: Used as a fluorescent tracer in live-cell imaging, super-resolution microscopy, and in vivo imaging due to its near-infrared emission.
• Pharmaceutical Research: Employed in drug discovery for target identification, high-throughput screening (HTS), and pharmacokinetic studies via fluorescence tracking.
• Material Science: Functionalization of nanoparticles and polymers to create advanced fluorescent materials for sensing and imaging applications.

Basic Information

Item	Detail
Product Name	Cy3.5-Cooh
CAS No.	1144107-79-8
Molecular Formula	C37H41N2O5S2+ (typically as a salt)
Molecular Weight	Contact for details
Synonyms	Cyanine3.5 carboxylic acid; Sulfo-Cyanine3.5 carboxylic acid; Cy3.5 Carboxylic Acid; 2-((1E,3E)-5-((E)-2-((1E,3E)-3-(3-Carboxypropyl)-5-chloro-1,1-dimethyl-1,3-dihydro-2H-benzo[e]indol-2-ylidene)ethylidene)-4-oxocyclopent-1-en-1-yl)vinyl)-1,1-dimethyl-3-(4-sulfobutyl)-1H-benzo[e]indol-3-ium; 1H-Benz[e]indolium, 2-[2-[2-chloro-3-[(1,3-dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclopenten-1-yl]ethenyl]-1,1-dimethyl-3-(4-carboxybutyl)-, inner salt
EINECS	Contact for details

Quality Control

Our Cy3.5-Cooh is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopy, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/emission profiles, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This product is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or desiccant after each use to maintain stability and performance.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms to reference standard
Identification (UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	~591 nm
Emission Maximum (λem)	~604 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, water (sulfonated form)
Functional Performance	Conjugation efficiency ≥ 90% (typical)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.