Description

Cyanine3 Carboxylic Acid CAS NO 1144107-76-5 is a reactive, near-infrared fluorescent dye derivative essential for advanced bioconjugation and labeling applications. Its core value lies in providing a stable, water-solubilizing functional group that enables the covalent attachment of the bright Cy3 fluorophore to biomolecules like proteins, peptides, and oligonucleotides. This product is critically needed by researchers and manufacturers in life sciences, diagnostic assay development, and pharmaceutical R&D for creating highly sensitive probes for fluorescence imaging, flow cytometry, and in situ hybridization.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of antibodies, proteins, peptides, and nucleic acids for fluorescence-based assays.
• Bioimaging and Microscopy: Development of fluorescent probes for cellular and tissue imaging, including confocal and super-resolution microscopy.
• Diagnostic Assay Development: Key component in manufacturing reagents for ELISA, lateral flow assays, and other in vitro diagnostic (IVD) kits.
• Flow Cytometry: Conjugation to antibodies or other ligands for cell surface marker detection and analysis.
• Fluorescence In Situ Hybridization (FISH): Labeling of oligonucleotide probes for genetic and genomic research applications.
• Drug Discovery & Development: Used in high-throughput screening (HTS) and pharmacokinetic studies to track biomolecule interactions.
• Material Science: Functionalization of nanoparticles and polymers for theranostic and sensing applications.

Basic Information

Product Name	Cyanine3 Carboxylic Acid
CAS No.	1144107-76-5
Molecular Formula	C34H39N2O4+
Molecular Weight	539.69 g/mol (as cation)
Synonyms	Cy3 Carboxylic Acid; Cy3-COOH; Sulfo-Cyanine3 carboxylic acid; Cy3 Acid; 1-(5-Carboxypentyl)-2-((1E,3E)-3-(1-(5-carboxypentyl)-3,3-dimethylindolin-2-ylidene)prop-1-en-1-yl)-3,3-dimethyl-3H-indol-1-ium; Cyanine 3 carboxylic acid; Cy3® Carboxylic Acid; Cy3 NHS Ester precursor
EINECS	Contact for details

Quality Control

Our Cyanine3 Carboxylic Acid is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. A detailed Certificate of Analysis (COA) documenting identity, purity, and absorbance/fluorescence properties is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under an inert atmosphere. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm-1M-1
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.