Description

Sulfo-Cyanine5 Carboxylic Acid is a near-infrared fluorescent dye derivative functionalized with a carboxylic acid group for bioconjugation. This water-soluble sulfonated cyanine dye is essential for creating stable, bright, and low-background fluorescent probes for advanced bioanalytical applications. It is primarily used by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for labeling proteins, antibodies, and other biomolecules.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of antibodies, proteins, peptides, and oligonucleotides via its carboxylic acid group for fluorescence detection.
• In Vivo and In Vitro Imaging: Used in the development of NIR fluorescent probes for deep-tissue imaging, tumor targeting, and cellular tracking due to reduced tissue autofluorescence.
• Diagnostic Assay Development: Key component in lateral flow assays (LFAs), enzyme-linked immunosorbent assays (ELISAs), and other immunoassays requiring high-sensitivity fluorescence detection.
• Flow Cytometry and Microscopy: Staining agent for cell surface markers and intracellular targets in multicolor flow cytometry and fluorescence microscopy applications.
• Drug Discovery & Development: Labeling tool for pharmacokinetic studies, receptor binding assays, and high-throughput screening (HTS) platforms.
• Nucleic Acid Detection: Labeling of DNA and RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.

Basic Information

Product Name	Sulfo-Cyanine5 Carboxylic Acid
CAS No.	1121756-16-8
Molecular Formula	C37H41N2O7S2+ (Counter ion typically provided)
Molecular Weight	Contact for details
Synonyms	Sulfo-Cy5 carboxylic acid; Cyanine5 sulfonated carboxylic acid; Sulfo-Cyanine5-COOH; Sulfo-Cy5-COOH; 2-((1E,3E)-5-((E)-1-(5-Carboxypentyl)-3,3-dimethyl-5-sulfoindolin-2-ylidene)penta-1,3-dien-1-yl)-1-ethyl-3,3-dimethyl-3H-indol-1-ium-5-sulfonate; Sulfo-Cy5 acid; Water-soluble Cy5 carboxylic acid; NIR dye carboxylic acid
EINECS	Contact for details

Quality Control

Our Sulfo-Cyanine5 Carboxylic Acid is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and absorbance/fluorescence spectroscopy for performance validation. Certificates of Analysis (COA) detailing all specifications are available upon request to ensure compliance with your research and manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥95.0% (Area %)
Absorption Maximum (λmax)	646 - 652 nm
Emission Maximum	662 - 668 nm
Extinction Coefficient (ε)	≥ 200,000 L·mol⁻¹·cm⁻¹
Water Content (KF)	≤ 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.