Description

n-Hydroxy Rhodamine B Amide is a chemically modified derivative of the classic Rhodamine B fluorophore, engineered with a reactive hydroxylamine group. This functionalization makes it a critical intermediate and labeling reagent for the synthesis of advanced fluorescent probes and sensors. It is primarily utilized by researchers and manufacturers in the life sciences, analytical chemistry, and material science sectors for applications requiring high-sensitivity detection and covalent conjugation.

Application

• Fluorescent Probe Synthesis: A key building block for creating custom fluorescent dyes and molecular beacons targeting specific biomolecules.
• Bioconjugation & Labeling: Used to covalently tag proteins, antibodies, nucleic acids, and other polymers with a bright, photostable red-emitting fluorophore.
• Chemical Sensing: Serves as a core structure in the development of "turn-on" or ratiometric fluorescent sensors for metal ions, reactive oxygen species, or pH.
• Cell Imaging & Microscopy: Enables the preparation of vital stains and organelle-specific trackers for confocal and super-resolution fluorescence microscopy.
• Material Science: Incorporated into polymers, nanoparticles, and silica matrices to create functional luminescent materials for optoelectronics or security inks.
• Diagnostic Assay Development: Employed in the R&D of lateral flow assays, ELISA, and other immunoassay platforms requiring stable fluorescent signal generation.

Basic Information

Product Name	n-Hydroxy Rhodamine B Amide
CAS No.	1115867-62-3
Molecular Formula	C28H30N3O4+ (typically as a salt, e.g., chloride or perchlorate)
Molecular Weight	472.56 g/mol (for free cation)
Synonyms	Rhodamine B Hydroxylamide; Rhodamine B Hydroxamic Acid; N-Hydroxyrhodamine B Amide; Rhodamine B N-Hydroxyamide; Hydroxylamine Rhodamine B derivative; Reactive Rhodamine B derivative; [9-(2-Carboxyphenyl)-6-diethylamino-3-xanthenylidene]-diethylammonium N-hydroxyamide; Fluorescent hydroxylamine reagent
EINECS	Contact for details

Quality Control

Our n-Hydroxy Rhodamine B Amide is produced under strict quality management protocols. Each batch undergoes comprehensive analytical characterization, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify key optical properties. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance is provided with every shipment to ensure consistency for your critical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. The compound is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider desiccation or storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder or crystalline solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95% (Standard Grade)
Absorption Max (λmax)	~550-570 nm
Emission Max (λem)	~575-595 nm
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.