Description

Azido-PEG12-NHS ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at one terminus and a click chemistry-ready azide group at the other. This compound is essential for creating stable, hydrophilic spacers in bioconjugation, enabling the precise attachment of biomolecules, labels, or surfaces under mild aqueous conditions. It is a critical reagent for researchers and manufacturers in biopharmaceutical development, proteomics, and advanced materials science who require reliable and efficient conjugation strategies.

Application

• Bioconjugation and ADC Development: Site-specific conjugation of antibodies, proteins, or peptides to drugs, toxins, or fluorescent dyes via click chemistry.
• PEGylation Chemistry: Introduction of a long, hydrophilic PEG12 spacer to enhance the solubility, stability, and pharmacokinetic properties of therapeutic molecules.
• Surface Functionalization: Modification of nanoparticles, biosensors, or microarray slides with azide groups for subsequent bio-orthogonal coupling.
• Polymer and Hydrogel Synthesis: Serving as a crosslinking agent or functional monomer in the creation of smart biomaterials and drug delivery systems.
• Chemical Biology Probes: Synthesis of activity-based probes or pull-down reagents for target identification and proteomic studies.
• Diagnostic Reagent Preparation: Labeling of antibodies or antigens for use in immunoassays (e.g., ELISA, lateral flow tests) to improve assay performance.

Basic Information

Product Name	Azido-PEG12-NHS Ester
CAS No.	1108750-59-9
Molecular Formula	C33H60N4O16
Molecular Weight	768.85 g/mol
Synonyms	NHS-PEG12-Azide; Azido-PEG12-NHS Ester; N3-PEG12-NHS; 2,5-Dioxopyrrolidin-1-yl 3-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-[2-(2-azidoethoxy)ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]ethoxy]propanoate; α-Azido-ω-NHS-PEG12; PEG12 Azide NHS Ester; Azido-dodecaethylene glycol-NHS ester
EINECS	Contact for details

Quality Control

Our Azido-PEG12-NHS Ester is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC, NMR, and MS, to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) with detailed specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term stability, store under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 90% of theoretical
Solubility	Soluble in DMSO, DMF, acetonitrile, and other polar aprotic solvents

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.