Description

Cy3 Diacid CAS NO 1034871-56-1 is a reactive, bifunctional cyanine dye derivative, serving as a critical intermediate for the synthesis of advanced fluorescent probes and labels. Its core value lies in enabling the creation of highly sensitive and stable conjugates for bioanalytical applications, where precise detection is paramount. This compound is essential for researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors who require reliable, high-purity building blocks for assay development and molecular imaging.

Application

• Fluorescent Probe Synthesis: Primary building block for creating Cy3-labeled antibodies, peptides, nucleic acids, and other biomolecules.
• Bioconjugation & Labeling: Used to covalently attach the Cy3 fluorophore to target molecules via its reactive carboxylic acid groups, enabling applications in flow cytometry (FACS), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH).
• Diagnostic Assay Development: Key component in the manufacture of reagents for ELISA, lateral flow assays, and other clinical diagnostic tests requiring high-sensitivity fluorescence detection.
• Bioimaging & Microscopy: Enables the preparation of contrast agents and labels for cellular and in vivo imaging techniques, including confocal and super-resolution microscopy.
• Material Science & Sensor Fabrication: Incorporated into polymers and nanomaterials to create fluorescent sensors for chemical or biological analytes.
• Life Science Research: Fundamental reagent for studying protein-protein interactions, nucleic acid dynamics, and cellular processes through fluorescence-based methodologies.

Basic Information

Product Name	Cy3 Diacid
CAS No.	1034871-56-1
Molecular Formula	C31H33N2O4+
Molecular Weight	497.61 g/mol
Synonyms	Cy3 Bis-Carboxylic Acid; Cy3(COOH)2; 1,1'-(3,3',3''-(2,5-Dioxopyrrolidine-1,3-diyl)bis(propane-3,1-diyl))bis(3,3-dimethyl-1-(5-carboxypentyl)-3H-indol-1-ium); Sulfo-Cyanine3 diacid; Cyanine3 Diacid; Cy3 NHS Ester precursor; Reactive Cy3 dye intermediate
EINECS	Contact for details

Quality Control

Our Cy3 Diacid is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify optical properties. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (MS)	Consistent with molecular ion [M]+
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm-1M-1
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.