Description

Biotin-4-Fluorescein is a high-purity, fluorescently labeled derivative of biotin, designed for advanced molecular detection and analysis. This compound is essential for creating highly sensitive and specific detection systems in life science research and diagnostic development. It is primarily used by researchers and manufacturers in the fields of biochemistry, molecular biology, and in vitro diagnostics who require reliable, high-affinity labeling reagents.

Application

• Fluorescent Labeling Agent: Used to label proteins, antibodies, or nucleic acids for detection in fluorescence microscopy, flow cytometry, and other imaging techniques.
• Streptavidin/Biotin System Enhancement: Serves as a critical component in ELISA, Western blotting, and immunohistochemistry, leveraging the strong biotin-streptavidin interaction for signal amplification.
• Diagnostic Assay Development: Integral to the development of fluorescent immunoassays and biosensors for clinical diagnostics and point-of-care testing.
• Cellular and Tissue Staining: Employed in fluorescence in situ hybridization (FISH) and other probe-based techniques for visualizing specific cellular targets.
• Drug Discovery Research: Utilized in high-throughput screening (HTS) and binding studies to track molecular interactions.
• Bioconjugation and Probe Synthesis: Acts as a versatile building block for synthesizing custom fluorescent probes and conjugates for targeted delivery studies.

Basic Information

Product Name	Biotin-4-Fluorescein
CAS No.	1032732-74-3
Molecular Formula	C34H32N4O7S
Molecular Weight	640.71 g/mol
Synonyms	5-((3aR,4S,6aR)-2-Oxo-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-4-yl)-N-(6-(2,7-dichloro-3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthen]-5-yl)hexyl)pentanamide; Biotin-4-fluorescein conjugate; Biotin-Fluorescein; Fluorescein Biotin; Biotinamidohexanoic acid fluorescein conjugate; Biotin-X-fluorescein; Fluorescein-labeled biotin; Biotin-fluorescein isothiocyanate (FITC) analog
EINECS	Contact for details

Quality Control

Our Biotin-4-Fluorescein is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized using advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and fluorescence properties. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient	~68,000 cm⁻¹M⁻¹ at 494 nm
Fluorescence Emission	~518 nm
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.