Description

Cyanine3 NHS Ester is a reactive, amine-reactive fluorescent labeling reagent widely used for the preparation of biological conjugates. Its core value lies in providing a bright, photostable red-fluorescent tag with excellent water solubility, enabling sensitive detection and imaging applications. This high-performance dye is essential for researchers and manufacturers in life sciences, diagnostics, and pharmaceutical development who require reliable and efficient protein, antibody, and oligonucleotide labeling.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to primary amines (-NH₂) on proteins, peptides, antibodies, and oligonucleotides for detection and analysis.
• Bioimaging and Microscopy: Preparation of fluorescent probes for cellular imaging, fluorescence in situ hybridization (FISH), and super-resolution microscopy techniques.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent immunoassays (e.g., ELISA, lateral flow assays) and other clinical diagnostic reagents.
• Flow Cytometry: Creation of antibody-dye conjugates for cell surface marker detection and immunophenotyping in flow cytometry applications.
• Drug Discovery & Bioconjugation: Used in the synthesis of antibody-drug conjugates (ADCs) and other targeted therapeutic agents for tracking and pharmacokinetic studies.
• Genomic and Proteomic Research: Labeling of nucleotides for microarray analysis or proteins for gel-based detection and western blotting.

Basic Information

Product Name	Cyanine3 NHS Ester
CAS No.	1032678-38-8
Molecular Formula	C₃₃H₃₉N₃O₄
Molecular Weight	541.69 g/mol
Synonyms	Cy3 NHS Ester; Cy3® NHS Ester; Sulfo-Cyanine3 NHS Ester (note: check sulfonation); 1-(6-((2,5-Dioxopyrrolidin-1-yl)oxy)-6-oxohexyl)-3,3-dimethyl-2-((E)-3-((E)-1,3,3-trimethylindolin-2-ylidene)prop-1-en-1-yl)-3H-indol-1-ium; Cy3 N-hydroxysuccinimide ester; NHS-Cy3; Cyanine 3 carboxylic acid N-succinimidyl ester
EINECS	Contact for details

Quality Control

Our Cyanine3 NHS Ester is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and spectrophotometry for labeling efficiency verification, to ensure it meets the high standards required for sensitive research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance, fluorescence, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.