Description

Cyanine3 Carboxylic Acid CAS NO 1032678-01-5 is a reactive, near-infrared fluorescent dye derivative essential for advanced bioconjugation and labeling applications. Its carboxylic acid functional group allows for efficient covalent attachment to biomolecules such as proteins, peptides, and antibodies, enabling high-sensitivity detection and imaging. This compound is a critical reagent for researchers and manufacturers in the fields of life sciences, diagnostic assay development, and pharmaceutical research, where precise and stable fluorescent labeling is required.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for use in immunofluorescence (IF), flow cytometry (FACS), and Western blotting.
• Diagnostic Assay Development: Key component in the synthesis of probes and reagents for in vitro diagnostics (IVD), including lateral flow assays and ELISA.
• Bioimaging and Microscopy: Preparation of fluorescent conjugates for cellular and tissue imaging, including confocal and super-resolution microscopy.
• Drug Discovery & Development: Used in high-throughput screening (HTS) and pharmacokinetic studies to label and track compounds of interest.
• Nucleic Acid Detection: Labeling of DNA and RNA probes for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Material Science: Functionalization of nanoparticles and polymers to create fluorescent materials for sensing and tracking.

Basic Information

Product Name	Cyanine3 Carboxylic Acid
CAS No.	1032678-01-5
Molecular Formula	C33H39N2O2+
Molecular Weight	495.68 g/mol
Synonyms	Cy3 Carboxylic Acid; Cy3-COOH; Sulfo-Cyanine3 carboxylic acid (non-sulfonated form); 1H-Benz[e]indolium, 2-[2-[2-chloro-3-[(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,3,3-trimethyl-, inner salt, carboxylic acid derivative; 2-[2-[2-Chloro-3-[(1,3-dihydro-1,3,3-trimethyl-2H-benz[e]indol-2-ylidene)ethylidene]-1-cyclohexen-1-yl]ethenyl]-1,3,3-trimethyl-1H-benz[e]indolium-5-carboxylic acid; Cyanine 3 acid; Cy3™ Carboxylic Acid (Lumiprobe® equivalent); Reactive Cy3 dye
EINECS	Contact for details

Quality Control

Our Cyanine3 Carboxylic Acid is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for absorbance/fluorescence verification, and identity confirmation by mass spectrometry (MS). Certificates of Analysis (COA) detailing all specifications are provided with every shipment. We support compliance with ISO 13485 standards for diagnostic and research use.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For optimal performance, store the product in the original container in a cool, dry, and dark place.

Specification

Item	Specification
Appearance	Dark green to purple solid or film
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	548 - 552 nm
Emission Maximum	562 - 568 nm
Extinction Coefficient (ε)	≥ 150,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.