Description

N,N'-Bis[2-(Acetamido)Ethyl]-N,N'-Dimethylrhodamine CAS NO 1022835-74-0 is a specialized rhodamine-based fluorescent dye derivative, engineered for advanced research and diagnostic applications. Its core value lies in its enhanced photostability and specific functionalization, making it a critical component for developing highly sensitive detection systems. This compound is essential for researchers and manufacturers in the fields of bioconjugation, flow cytometry, and fluorescence microscopy, where precise and reliable labeling is paramount.

Application

• Fluorescent Labeling and Bioconjugation: Used to tag antibodies, proteins, peptides, and nucleic acids for detection and imaging.
• Flow Cytometry: Serves as a vital fluorescent probe for cell sorting, immunophenotyping, and intracellular marker analysis.
• Fluorescence Microscopy & Imaging: Enables high-resolution visualization of cellular structures and dynamic processes in live and fixed cells.
• Diagnostic Assay Development: A key reagent in the formulation of ELISA kits, lateral flow assays, and other in-vitro diagnostic (IVD) products.
• Chemical Sensing and Probe Design: Acts as a signaling moiety in the development of sensors for ions, small molecules, and enzymatic activity.
• Materials Science: Incorporated into polymers and nanoparticles to create functional, fluorescent materials for tracking and analysis.

Basic Information

Product Name	N,N'-Bis[2-(Acetamido)Ethyl]-N,N'-Dimethylrhodamine
CAS No.	1022835-74-0
Molecular Formula	C31H33N5O4
Molecular Weight	539.63 g/mol
Synonyms	N,N'-Bis(2-acetamidoethyl)-N,N'-dimethylrhodamine; BADE-DMRhodamine; Rhodamine B bis(2-acetamidoethyl) dimethyl derivative; 2-[6-(Dimethylamino)-3-(dimethyliminio)-3H-xanthen-9-yl]-5-[2-(2-acetamidoethyl)(methyl)amino]benzoate; Fluorescent Xanthene Dye Derivative
EINECS	Contact for details

Quality Control

Every batch of N,N'-Bis[2-(Acetamido)Ethyl]-N,N'-Dimethylrhodamine is manufactured under strict quality management protocols. We employ advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and batch-to-batch consistency suitable for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing all specifications is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. Due to its hygroscopic nature, the container should be kept in a desiccated environment. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines
Fluorescence Properties	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.