Description

Pyridoxal-5'-Phosphate-6-(2'-Naphthylazo-6'-Nitro-4',8'-Disulfonate) Tetrasodium Salt is a chemically modified, water-soluble derivative of the biologically essential coenzyme pyridoxal 5'-phosphate (PLP). This compound is specifically engineered as a chromogenic substrate or probe for the detection and study of enzymatic activity, particularly for transaminases and other PLP-dependent enzymes. It is a critical reagent for researchers and manufacturers in diagnostic assay development, biochemical research, and high-throughput screening platforms where precise and sensitive enzyme detection is required.

Application

• Clinical Diagnostic Reagents: Core component in enzymatic assay kits for the quantitative measurement of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and other transaminases in serum.
• Biochemical Research: Used as a chromogenic probe to study the kinetics, inhibition, and mechanism of PLP-dependent enzymes in vitro.
• Pharmaceutical Screening: Employed in high-throughput screening (HTS) assays to identify potential drug candidates that modulate aminotransferase activity.
• Food & Beverage Analysis: Applied in enzymatic methods for quality control and process monitoring in fermentation and food production industries.
• Veterinary Diagnostics: Utilized in veterinary medicine for liver function panels and disease diagnosis in animals.
• Academic & Institutional Research: Essential for university and government laboratories conducting fundamental research on vitamin B6 metabolism and enzyme function.

Basic Information

Product Name	Pyridoxal-5'-Phosphate-6-(2'-Naphthylazo-6'-Nitro-4',8'-Disulfonate) Tetrasodium Salt
CAS No.	1021868-77-8
Molecular Formula	C21H13N4Na4O16PS2
Molecular Weight	806.37 g/mol (anhydrous basis)
Synonyms	PLP-ANSA; Pyridoxal Phosphate ANSA Salt; 6-[(2-Naphthylazo)-6-nitro-4,8-disulfonate]-pyridoxal-5'-phosphate tetrasodium salt; P-5'-P-ANSA; ALT/AST Substrate; Chromogenic PLP Derivative; Transaminase Substrate; Vitamin B6 Phosphate Azo Dye
EINECS	Contact for details

Quality Control

Our production of this specialized biochemical adheres to strict quality management systems. Each batch is characterized and tested to ensure high purity, consistent performance, and lot-to-lot reproducibility suitable for diagnostic and research applications. Certificates of Analysis (COA) detailing identity, purity, absorbance characteristics, and functional performance in standard assay systems are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This material is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, consider storage under desiccant. Allow the sealed container to reach room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Orange to red-brown powder
Identification (HPLC/UV-Vis)	Conforms to reference standard
Assay (HPLC)	≥ 95.0%
Absorbance Maximum (λmax)	505 - 515 nm (in buffer)
Extinction Coefficient (ε)	Contact for details
Water Content (KF)	≤ 5.0%
Solubility	Freely soluble in water, buffer solutions
Functional Activity	Validated in standard transaminase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.