Description

5-Tamra Azide is a high-purity, functionalized fluorescent dye derivative designed for advanced bioconjugation and labeling applications. This compound is critical for researchers and developers requiring precise, covalent attachment of a bright, orange-red fluorescent tag to biomolecules via click chemistry. It is primarily utilized in the life sciences, diagnostics, and pharmaceutical research sectors for applications such as bioimaging, flow cytometry, and the development of fluorescent probes and sensors.

Application

• Bioorthogonal Labeling: Used in copper-catalyzed azide-alkyne cycloaddition (CuAAC) click chemistry to fluorescently label proteins, antibodies, peptides, and oligonucleotides.
• Live-Cell Imaging: Enables real-time visualization and tracking of cellular processes and biomolecule localization with high sensitivity.
• Flow Cytometry & FACS: Serves as a bright fluorophore for cell surface marker staining and intracellular target detection.
• Fluorescent Probe Development: A key building block for creating custom activity-based probes and biosensors.
• Diagnostic Assays: Incorporated into lateral flow assays, ELISA, and other immunoassay formats for detection.
• Super-Resolution Microscopy: Suitable for advanced imaging techniques like STORM/PALM due to its photophysical properties.
• Material Science: Functionalization of polymers, nanoparticles, and surfaces for traceable material studies.

Basic Information

Product Name	5-Tamra Azide
CAS No.	1006592-61-5
Molecular Formula	C25H20N6O5
Molecular Weight	484.47 g/mol
Synonyms	5-Carboxytetramethylrhodamine Azide; 5-TAMRA Azide; 5-TAMRA N3; Tetramethylrhodamine-5-azide; 6-Carboxy-tetramethylrhodamine-azide; TAMRA-5-Azide; Azido-TAMRA; 5-isomer TAMRA Azide
EINECS	Contact for details

Quality Control

Every batch of 5-Tamra Azide is manufactured under strict quality management systems and undergoes comprehensive analytical testing to ensure high purity and batch-to-batch consistency. Our products are characterized by HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) providing purity, identity, and impurity profile is supplied with each shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95%
Absorption (λmax)	~540-555 nm
Emission (λmax)	~565-580 nm
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.