Description

n-Hydroxysulfosuccinimidyl-4-Azidobenzoate is a heterobifunctional crosslinking reagent featuring an amine-reactive N-hydroxysulfosuccinimide (sulfo-NHS) ester and a photoactivatable aryl azide group. This dual functionality is critical for creating stable covalent bonds between biomolecules under mild, aqueous conditions, enabling precise conjugation strategies. It is an essential tool for researchers and developers in bioconjugation, proteomics, and advanced materials science, particularly for photoaffinity labeling and the development of diagnostic assays and immobilized ligands.

Application

• Photoaffinity Labeling: Used to covalently tag and identify protein-ligand interactions or receptor binding sites upon UV light activation.
• Surface Immobilization: For the covalent attachment of peptides, proteins, or other amine-containing biomolecules to solid supports (e.g., biosensor chips, microarray slides, beads) to create bioactive surfaces.
• Antibody-Drug Conjugate (ADC) & Bioconjugation Research: Serves as a key reagent in the development and study of novel conjugation strategies linking antibodies, proteins, or peptides to drugs, dyes, or other molecules.
• Hydrogel and Biomaterial Fabrication: Employed in crosslinking polymers or modifying biomaterials to introduce specific biological functionalities for tissue engineering and drug delivery systems.
• Diagnostic Assay Development: Facilitates the stable conjugation of capture antibodies or antigens to plates or particles in ELISA and other immunoassay formats.
• Protein-Protein Crosslinking Studies: Enables the investigation of protein complexes and interactions in structural biology and molecular biology research.

Basic Information

Product Name	n-Hydroxysulfosuccinimidyl-4-Azidobenzoate
CAS No.	199804-22-3
Molecular Formula	C11H8N4O7S
Molecular Weight	340.27 g/mol
Synonyms	Sulfo-NHS-4-Azidobenzoate; 4-Azidobenzoic Acid N-Hydroxysulfosuccinimide Ester; Sulfo-SANPAH; N-Sulfosuccinimidyl 4-Azidobenzoate; 4-Azidobenzoyloxysulfosuccinimide; NHS-4-Azidobenzoate, Sulfonated; Heterobifunctional Crosslinker, Sulfo-NHS-Azide; 4-Azido-benzoic acid 2,5-dioxo-1-pyrrolidinyl ester 1-sulfonic acid
EINECS	Contact for details

Quality Control

Our n-Hydroxysulfosuccinimidyl-4-Azidobenzoate is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research and development applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. A detailed Certificate of Analysis (COA) providing lot-specific data is available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0 %
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.