Description

5(6)-Carboxy-X-Rhodamine is a high-purity, reactive fluorescent dye derivative of the X-rhodamine family, widely recognized for its bright red-orange emission and excellent photostability. This compound is specifically engineered for fluorescence labeling and detection applications, offering superior performance in demanding analytical and imaging environments. It is an essential reagent for researchers and manufacturers in the fields of life sciences, diagnostics, and analytical chemistry who require reliable, high-quality fluorescent probes.

Application

• Fluorescent Labeling of Biomolecules: Covalent conjugation to proteins, antibodies, peptides, and nucleic acids for use in fluorescence microscopy, flow cytometry, and immunoassays.
• Bioconjugation & Probe Synthesis: A key intermediate for synthesizing custom fluorescent probes, sensors, and molecular beacons due to its carboxylic acid functional group.
• Live-Cell and Fixed-Cell Imaging: Provides bright, photostable signals for tracking cellular processes, protein localization, and dynamic events in biological research.
• Diagnostic Assay Development: Used as a detection label in ELISA, lateral flow assays, and other in vitro diagnostic (IVD) platforms.
• Fluorescence In Situ Hybridization (FISH): Serves as a fluorescent tag for nucleic acid probes in genetic testing and cytogenetics.
• High-Throughput Screening (HTS): Employed in assay development for drug discovery and biochemical analysis where a red-shifted fluorophore is required.

Basic Information

Product Name	5(6)-Carboxy-X-Rhodamine
CAS No.	198978-94-8
Molecular Formula	C34H31N2O7
Molecular Weight	579.62 g/mol
Synonyms	5(6)-Carboxy-X-rhodamine; 5(6)-Carboxy-X-rhodamine, mixed isomers; 5- and 6-Carboxy-X-rhodamine; X-rhodamine-5(6)-carboxylic acid; 5-Carboxy-X-rhodamine; 6-Carboxy-X-rhodamine; ROX acid; CXR
EINECS	Contact for details

Quality Control

Our 5(6)-Carboxy-X-Rhodamine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for absorbance/emission verification, and functional testing in conjugation assays. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0% (sum of isomers)
Absorption Maximum (λmax)	575 - 585 nm
Emission Maximum	600 - 610 nm
Extinction Coefficient (ε)	≥ 80,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 3.0 %
Functional Performance	Conjugation efficiency ≥ 90%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.