Description

1,4-Dichloro 5-Carboxytetramethylrhodamine is a high-purity, functionalized derivative of the widely used tetramethylrhodamine (TAMRA) fluorophore. This compound matters for its enhanced photostability and reactive carboxylic acid group, which enables efficient covalent conjugation to biomolecules like proteins, peptides, and oligonucleotides. It is primarily needed by researchers and manufacturers in the biotechnology, diagnostics, and pharmaceutical industries for developing advanced fluorescent probes, assays, and labeling kits.

Application

• Fluorescent Labeling Reagent: Covalent conjugation to antibodies, proteins, and other biomolecules via its carboxylic acid group for fluorescence microscopy (FLM), flow cytometry, and immunoassays.
• Nucleic Acid Probes: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH), real-time PCR (qPCR), and other molecular diagnostic applications.
• Sensor Development: Acting as a core fluorophore in the design of chemical sensors and indicators for detecting various analytes.
• Material Science: Incorporation into polymers or nanoparticles to create fluorescent materials with specific optical properties.

Basic Information

Item	Details
Product Name	1,4-Dichloro 5-Carboxytetramethylrhodamine
CAS No.	198546-47-3
Molecular Formula	C27H21Cl2N2O5
Molecular Weight	524.37 g/mol
Synonyms	5-Carboxy-1,4-dichlorotetramethylrhodamine; 5(6)-Carboxy-1,4-dichloro-TAMRA; 1,4-Dichloro-5(6)-carboxytetramethylrhodamine; 1,4-Dichloro-5-carboxy-TAMRA; 5-Carboxy-1,4-dichloro-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-3',6'-bis(N,N-dimethylacetamide); Dichloro Carboxy TAMRA; DCTMR-COOH
EINECS	Contact for details

Quality Control

Our 1,4-Dichloro 5-Carboxytetramethylrhodamine is produced under strict quality management protocols to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic identification (NMR, MS). Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This material is light-sensitive (store away from light). For long-term storage, consider desiccation to maintain stability.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms
Purity (HPLC)	≥ 95% (Area %)
Absorption (λmax)	Contact for details
Emission (λmax)	Contact for details
Extinction Coefficient	Contact for details
Water Content (KF)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.