Description

2',7'-Difluoro-3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthene]-5-Carboxylic Acid 2,5-Dioxo-1-Pyrrolidinyl Ester is a high-purity, reactive chemical intermediate, specifically an activated ester derivative of a fluorescein-based dye. Its core value lies in its ability to serve as a versatile labeling reagent for the covalent attachment of fluorescent probes to biomolecules, enabling sensitive detection and analysis. This compound is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require reliable, high-performance reagents for assay development and bio-conjugation.

Application

• Fluorescent Labeling Reagent: Primary use as an activated ester for covalently tagging proteins, antibodies, peptides, and nucleic acids with a fluorophore.
• Diagnostic Assay Development: Key component in the synthesis of fluorescent probes and tracers for ELISA, lateral flow assays, and other immunoassay platforms.
• Bioconjugation Chemistry: Enables the creation of stable amide bonds with primary amine groups (-NH2) on target molecules under mild aqueous conditions.
• Cell Imaging and Microscopy: Used to prepare fluorescent conjugates for cellular staining, flow cytometry (FACS), and fluorescence microscopy applications.
• Biomedical Research: Facilitates the study of biomolecular interactions, localization, and dynamics through fluorescence-based techniques.
• Sensor and Probe Manufacturing: Intermediate in the production of advanced chemical sensors and molecular probes for research and clinical diagnostics.

Basic Information

Item	Details
Product Name	2',7'-Difluoro-3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthene]-5-Carboxylic Acid 2,5-Dioxo-1-Pyrrolidinyl Ester
CAS No.	198139-51-4
Molecular Formula	C27H13F2NO9
Molecular Weight	533.39 g/mol
Synonyms	Oregon Green™ 488 NHS Ester (Isomer); 5-Carboxyfluorescein Diacetate Succinimidyl Ester, Difluoro derivative; 2',7'-Difluorofluorescein-5(6)-Carboxamidocaproic Acid N-Succinimidyl Ester; 6-FAM, SE, Difluoro; 5(6)-FAM, SE, Difluoro; 5-(and-6)-Carboxyfluorescein Diacetate Succinimidyl Ester, 2',7'-Difluoro; 2',7'-Difluoro-5(6)-carboxyfluorescein N-succinimidyl ester; 2',7'-Difluoro-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid 2,5-dioxopyrrolidin-1-yl ester
EINECS	Contact for details

Quality Control

Our 2',7'-Difluoro-3',6'-Dihydroxy-3-Oxospiro[Isobenzofuran-1(3H),9'-[9H]Xanthene]-5-Carboxylic Acid 2,5-Dioxo-1-Pyrrolidinyl Ester is manufactured under strict quality systems to ensure high purity and batch-to-batch consistency, critical for reproducible bio-conjugation results. All batches undergo comprehensive analytical testing including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, confirming identity, purity, and suitability for sensitive life science applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term stability, store in a freezer and allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Keep the container tightly sealed in a dry and well-ventilated place.

Specification

Item	Specification
Appearance	Orange to red solid or powder
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0% (Area Percent)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.