Description

5,6-Carboxyfluorescein Dipivalate Succinimide Ester is a high-purity, amine-reactive fluorescent labeling reagent. This compound is critical for creating stable, fluorescently tagged biomolecules, enabling sensitive detection and analysis. It is primarily needed by researchers and manufacturers in the life sciences, diagnostics, and pharmaceutical development sectors for applications such as flow cytometry, immunoassays, and nucleic acid labeling.

Application

• Fluorescent Labeling of Proteins and Antibodies: Conjugation to primary amines (e.g., lysine residues) for use in immunofluorescence (IF), flow cytometry, and Western blotting.
• Nucleic Acid Probe Synthesis: Labeling of oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Cell Tracing and Viability Assays: Intracellular labeling for tracking cell proliferation, migration, and cytotoxicity studies.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent lateral flow assays and ELISA kits.
• Microscopy and Imaging: Used to prepare fluorescent conjugates for confocal and super-resolution microscopy.
• Bioconjugation and Chemical Biology: A versatile building block for creating custom fluorescent probes and drug conjugates.

Basic Information

Product Name	5,6-Carboxyfluorescein Dipivalate Succinimide Ester
CAS No.	197850-75-2
Molecular Formula	C41H37NO13
Molecular Weight	751.73 g/mol
Synonyms	5(6)-Carboxyfluorescein Di-pivalate N-Succinimidyl Ester; 5(6)-CFDA, SE; 5(6)-Carboxyfluorescein Diacetate Succinimidyl Ester (common misnomer); CFDA, SE; 5,6-CFDA-SE; 6-Carboxyfluorescein Di(pivaloyloxymethyl) Ester N-Hydroxysuccinimide Ester; Fluorescein-based NHS Ester; Amine-reactive Fluorescein Derivative
EINECS	Contact for details

Quality Control

Our 5,6-Carboxyfluorescein Dipivalate Succinimide Ester is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive research and in vitro diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and functional performance is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Single Isomer Content (HPLC)	≥ 90.0%
Extinction Coefficient (ε)	~80,000 cm⁻¹M⁻¹ at 492 nm (pH 9)
Water Content (KF)	≤ 1.0%
Functional Performance	Conjugation efficiency ≥ 90% (per internal test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.