Description

2',7'-Difluorofluorescein CAS NO 195136-58-4 is a chemically modified derivative of fluorescein, where fluorine atoms are introduced at specific positions on the aromatic rings. This modification significantly enhances its photostability and alters its spectral properties, making it a superior fluorophore for demanding analytical applications. It is primarily utilized by researchers and manufacturers in the life sciences, diagnostics, and chemical synthesis sectors who require high-performance fluorescent tags and probes.

Application

• Fluorescent Labeling Agent: Used as a high-stability fluorescent tag for biomolecules such as proteins, antibodies, and nucleic acids in assay development.
• Biochemical Probes: Serves as a core structure for synthesizing advanced fluorescent probes and sensors for detecting enzymes, ions, or pH changes.
• Diagnostic Reagents: A key component in the formulation of reagents for fluorescence-based diagnostic kits and immunoassays.
• Cell Staining & Microscopy: Employed in cellular imaging and flow cytometry for visualizing cellular structures and processes with reduced photobleaching.
• Chemical Research Intermediate: Acts as a crucial synthetic intermediate for developing novel xanthene dyes and other specialized fluorophores.
• Material Science Marker: Incorporated into polymers or nanoparticles to create fluorescent materials for tracking and sensing applications.

Basic Information

Product Name	2',7'-Difluorofluorescein
CAS No.	195136-58-4
Molecular Formula	C20H10F2O5
Molecular Weight	368.29 g/mol
Synonyms	2',7'-Difluorofluorescein; 3',6'-Dihydroxy-2',7'-difluoro-3H-spiro[isobenzofuran-1,9'-xanthen]-3-one; 2',7'-Difluoro-3',6'-dihydroxyfluoran; DFF; 2,7-Difluorofluorescein; Fluorescein, 2',7'-difluoro-; 2',7'-Difluor-fluorescein
EINECS	Contact for details

Quality Control

Our 2',7'-Difluorofluorescein is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity assessment and spectroscopic methods for identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Loss on Drying	≤ 2.0%
Fluorescence Properties	Conforms to reference

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.