Description

Oregon Green 488 Carboxylic Acid is a high-purity, fluorescent dye derivative widely used as a versatile labeling reagent. Its core value lies in its bright green fluorescence, excellent photostability, and the reactive carboxylic acid group that facilitates covalent conjugation to biomolecules. This product is essential for researchers and manufacturers in the life sciences, diagnostics, and biotechnology sectors who require reliable fluorescent probes for labeling proteins, peptides, antibodies, and other amine-containing targets.

Application

• Biomolecule Labeling: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for fluorescence-based assays (e.g., ELISA, Western blotting, flow cytometry).
• Fluorescent Probe Synthesis: Serving as a key intermediate for creating custom fluorescent sensors, enzyme substrates, and molecular beacons.
• Cell Biology & Imaging: Labeling of cellular components for live-cell imaging, tracking, and intracellular localization studies.
• Diagnostic Assay Development: Integration into lateral flow assays, microarrays, and other diagnostic platforms requiring stable fluorescent signals.
• Material Science: Functionalization of nanoparticles, polymers, and surfaces to impart fluorescence for tracking and analysis.

Basic Information

Product Name	Oregon Green 488 Carboxylic Acid
CAS No.	195136-52-8
Molecular Formula	C₂₁H₁₅F₂NO₇
Molecular Weight	431.34 g/mol
Synonyms	OG 488 carboxylic acid; 6-Carboxyfluorescein diacetate, 2',7'-difluoro-; 2',7'-Difluoro-6-carboxyfluorescein diacetate; 5(6)-Carboxy-2',7'-difluorofluorescein diacetate; Oregon Green™ 488 carboxylic acid; 6-FAM diacetate, 2',7'-difluoro-; 6-Carboxy-2',7'-difluorofluorescein diacetate; 3',6'-Diacetyloxy-2',7'-difluoro-6-carboxy-spiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one
EINECS	Contact for details

Quality Control

Our Oregon Green 488 Carboxylic Acid is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high performance. Each lot is characterized using advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to confirm identity, purity, and fluorescence properties. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep the container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Excitation Maximum (λex)	~ 496 nm
Emission Maximum (λem)	~ 524 nm
Extinction Coefficient (ε)	~ 80,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 3.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.