Description

6-Carboxy-X-Rhodamine is a high-purity, reactive derivative of the X-rhodamine fluorophore, widely recognized for its excellent photostability and bright fluorescence. This compound is a critical intermediate for synthesizing advanced fluorescent probes, labels, and conjugates used in cutting-edge life science research and diagnostics. It is essential for researchers and manufacturers in the fields of bioimaging, flow cytometry, nucleic acid detection, and immunoassay development who require reliable, high-performance fluorescent tags.

Application

• Fluorescent Labeling Reagent: Used for the covalent labeling of proteins, antibodies, peptides, and oligonucleotides to create sensitive detection probes.
• Nucleic Acid Staining & Detection: Serves as an active ester for preparing fluorescent DNA/RNA probes for techniques like FISH (Fluorescence In Situ Hybridization) and real-time PCR.
• Bioconjugation & Probe Synthesis: A key building block for developing custom fluorescent conjugates and molecular beacons for cellular and molecular imaging.
• Flow Cytometry & Microscopy: Enables the creation of bright, photostable antibody-dye conjugates for multicolor flow cytometry and high-resolution fluorescence microscopy.
• Diagnostic Assay Development: Incorporated into lateral flow assays, ELISA, and other diagnostic platforms as a signal-generating component.
• Material Science & Sensor Development: Used in the fabrication of fluorescent sensors and functional materials for chemical and biological sensing applications.

Basic Information

Product Name	6-Carboxy-X-Rhodamine
CAS No.	194785-18-7
Molecular Formula	C33H31N2O7
Molecular Weight	567.61 g/mol
Synonyms	6-ROX; 6-Carboxy-X-rhodamine; X-Rhodamine-6-carboxylic acid; 6-Carboxyrhodamine X; 6-Carboxy-X-rhodamine acid; ROX-6; 6-Carboxy-X-rhodamine, SE (derivative); 6-Carboxy-X-rhodamine succinimidyl ester (derivative)
EINECS	Contact for details

Quality Control

Our 6-Carboxy-X-Rhodamine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry for optical property verification. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with your research and production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a temperature between -20°C to -15°C for long-term stability. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	575 - 585 nm
Emission Maximum	595 - 605 nm
Extinction Coefficient (ε)	≥ 80,000 cm⁻¹M⁻¹
Water Content (KF)	≤ 3.0%
Solubility	Soluble in DMSO, DMF, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.