Description

n-Alloc-L-Glutamic Acid 5-tert-Butyl Ester is a high-purity, protected amino acid derivative essential for advanced peptide synthesis. This compound serves as a critical building block, offering orthogonal protection strategies for the side-chain carboxylic acid and α-amino groups of glutamic acid. It is primarily utilized by research institutions and pharmaceutical companies engaged in the development of novel therapeutic peptides, peptidomimetics, and biochemical probes. The tert-butyl ester and Alloc (allyloxycarbonyl) protecting groups provide selective deprotection pathways, enabling complex, multi-step synthetic sequences with high fidelity.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key intermediate for the controlled incorporation of glutamic acid residues with orthogonal side-chain protection.
• Solution-Phase Peptide Chemistry: Used in the synthesis of complex peptide fragments and cyclic peptides where selective deprotection is required.
• Pharmaceutical Research & Development: Serves as a building block for creating peptide-based drug candidates, including hormone analogs and enzyme inhibitors.
• Bioconjugation and Probe Development: Facilitates the site-specific modification of peptides for labeling, imaging, or creating antibody-drug conjugates (ADCs).
• Combinatorial Chemistry Libraries: Employed in the generation of diverse peptide libraries for high-throughput screening against biological targets.
• Academic Research: Used in studies exploring protein structure-function relationships, peptide self-assembly, and novel synthetic methodologies.

Basic Information

Product Name	n-Alloc-L-Glutamic Acid 5-tert-Butyl Ester
CAS No.	192753-43-8
Molecular Formula	C₁₃H₂₁NO₆
Molecular Weight	287.31 g/mol
Synonyms	L-Glutamic acid, N-[(allyloxy)carbonyl]-, 5-(1,1-dimethylethyl) ester; N-Alloc-L-glutamic acid 5-tert-butyl ester; N-Alloc-Glu(OtBu)-OH; (2S)-2-{[(Allyloxy)carbonyl]amino}pentanedioic acid 5-tert-butyl ester; Fmoc-Glu(OtBu)-OH related intermediate; Alloc-Glu(OtBu)-OH; Protected L-glutamic acid derivative
EINECS	Contact for details

Quality Control

Our n-Alloc-L-Glutamic Acid 5-tert-Butyl Ester is manufactured under strict quality control protocols to ensure batch-to-batch consistency and high chemical purity suitable for sensitive synthetic applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, chiral integrity, and impurity profiles. We adhere to cGMP guidelines where applicable to support our clients' regulatory and research requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (typically 15-25°C). This material is moisture-sensitive (hygroscopic); ensure the container is sealed under an inert atmosphere after opening to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Optical Rotation [α]²⁰D	Contact for details
Chiral Purity (HPLC)	≥99.0% (L-isomer)
Water Content (KF)	≤0.5%
Heavy Metals	≤10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.