Description

6-Carboxyfluorescein Dipivalate is a chemically protected derivative of the widely used fluorescent dye, fluorescein. This compound is specifically designed to be cell-permeable and non-fluorescent until intracellular esterases cleave the pivaloyl groups, releasing the highly fluorescent 6-carboxyfluorescein. It is an essential tool for researchers in cell biology and biochemistry who require precise, intracellular tracking and viability assays. The product is supplied as a high-purity material suitable for sensitive diagnostic and research applications.

Application

• Intracellular Esterase Activity Assays: Used as a substrate to measure cell viability and metabolic activity, where cleavage by nonspecific intracellular esterases generates fluorescent signal.
• Cell Permeability and Tracing Studies: Employed to track cell migration, proliferation, and cytoplasmic labeling due to its membrane-permeable, non-fluorescent pro-dye form.
• Flow Cytometry and Fluorescence Microscopy: Serves as a vital fluorescent probe for analyzing cell populations and visualizing intracellular processes in real-time.
• Drug Delivery and Nanoparticle Research: Utilized as a model cargo or tracer to study the efficiency of drug delivery systems and nanoparticle uptake mechanisms.
• Apoptosis and Cytotoxicity Testing: Applied in high-throughput screening assays to evaluate the effects of pharmaceutical compounds on cell health.
• Molecular Biology Reagents: Incorporated into kits and reagents for cell-based assays that require a sensitive and reliable fluorescent readout.

Basic Information

Product Name	6-Carboxyfluorescein Dipivalate
CAS No.	192374-17-7
Molecular Formula	C35H34O10
Molecular Weight	614.64 g/mol
Synonyms	6-CFDA; 6-Carboxyfluorescein Diacetate, Pivalate Mixed Isomers; 6-Carboxyfluorescein Di(pivaloyloxymethyl) Ester; CFDA; 5(6)-Carboxyfluorescein Diacetate Pivalate; 3',6'-Di-O-pivaloyl-5(6)-carboxyfluorescein; Fluorescein Derivative 6-CFDA; 6-CFDPA
EINECS	Contact for details

Quality Control

Our 6-Carboxyfluorescein Dipivalate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/IR)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.