Description

Fura-2Ff is a high-purity, cell-permeant fluorescent calcium indicator widely used in life science research. Its core value lies in enabling precise, real-time measurement of intracellular calcium ion (Ca²⁺) concentrations, a critical parameter in cellular signaling studies. This compound is essential for researchers in neurobiology, cardiology, and pharmacology who require reliable tools for studying calcium dynamics in live cells and tissues. The product is supplied under stringent quality control to ensure consistent performance and reliable experimental results.

Application

• Intracellular Calcium Imaging: Primary application for monitoring real-time Ca²⁺ flux in live cells using fluorescence microscopy (e.g., confocal, epifluorescence).
• Neuroscience Research: Studying calcium signaling in neurons, synaptic transmission, and neuronal activity.
• Cardiovascular Research: Investigation of calcium handling in cardiomyocytes, muscle contraction, and related pathophysiology.
• Drug Discovery & Pharmacology: High-throughput screening (HTS) assays to evaluate compound effects on cellular calcium pathways.
• Cell Physiology Studies: Fundamental research into calcium's role in cell cycle, apoptosis, secretion, and other cellular processes.
• Fluorometric Plate Reader Assays: Used in quantitative, population-based calcium measurement assays in multi-well formats.

Basic Information

Product Name	Fura-2Ff
CAS No.	192140-58-2
Molecular Formula	C₂₉H₂₈N₂O₁₃
Molecular Weight	612.54 g/mol
Synonyms	Fura-2Ff, Fura 2Ff, Fura2Ff, Fura-2FF, 1-[2-(5-Carboxyoxazol-2-yl)-6-aminobenzofuran-5-oxyl]-2-(2'-amino-5'-methylphenoxy)ethane-N,N,N',N'-tetraacetic acid, Fura-2FF pentapotassium salt (common salt form), Fura-2FF AM (acetoxymethyl ester), Cell-permeant calcium indicator Fura-2Ff
EINECS	Contact for details

Quality Control

Our Fura-2Ff is manufactured and tested to meet the exacting standards required for sensitive life science applications. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and performance-related specifications is provided with every shipment to ensure traceability and quality assurance for your research.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic (moisture-sensitive) nature, the container should be allowed to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Fluorescence Properties	Ex/Em ~ 340-380/510 nm (Ca²⁺ bound/free)
Solubility	Soluble in DMSO, DMF; aqueous buffer (as salt)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.