Description

n-D-Biotinyl-7-Amino-4-Methylcoumarin is a high-purity, fluorescent-labeled biotin derivative designed for advanced biochemical research and diagnostics. This compound is critical for developing highly sensitive detection and quantification assays due to its strong fluorescence and high-affinity biotin-streptavidin binding. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic industries for applications in fluorescence microscopy, immunoassays, and nucleic acid detection.

Application

• Fluorescence Labeling Agent: Used as a fluorescent probe for labeling proteins, antibodies, and nucleic acids in biochemical research.
• Diagnostic Assay Development: A key component in the formulation of sensitive ELISA, Western blot, and other immunoassay kits.
• Cell Imaging & Microscopy: Employed in fluorescence microscopy and flow cytometry for visualizing and tracking biomolecules within cells.
• Streptavidin/Biotin Technology: Serves as a critical linker in avidin-biotin complex (ABC) techniques, amplifying detection signals.
• Drug Discovery Research: Utilized in high-throughput screening (HTS) and target identification studies involving biotinylated molecules.
• Molecular Biology: Applied in fluorescence in situ hybridization (FISH) and other nucleic acid detection methodologies.

Basic Information

Product Name	n-D-Biotinyl-7-Amino-4-Methylcoumarin
CAS No.	191223-35-5
Molecular Formula	C25H32N4O5S
Molecular Weight	500.61 g/mol
Synonyms	7-Amino-4-methylcoumarin-biotin; Biotin-AMC; Biotinylated 7-Amino-4-Methylcoumarin; N-((6aS,9S,10aR)-10a-Hydroxy-6a-methyl-3-oxo-2,3,5,6,6a,9,10,10a-octahydrooxireno[2',3':9,10]pyrano[4,3-g]isoquinolin-9-yl)-7-(biotinylamino)-4-methyl-2H-chromen-2-one; AMC-Biotin; Biotin-7-Amino-4-Methylcoumarin; Fluorescent Biotin Conjugate
EINECS	Contact for details

Quality Control

Our n-D-Biotinyl-7-Amino-4-Methylcoumarin is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and diagnostic applications. Certificates of Analysis (COA) with detailed specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.