Description

2-Hydroxy Nile Red is a chemically modified derivative of the classic fluorescent dye Nile Red, designed to offer enhanced photophysical properties and improved solubility for advanced analytical applications. This compound matters for researchers and industrial scientists who require a highly sensitive and versatile probe for detecting and quantifying lipids, biomolecules, and hydrophobic environments. It is essential for professionals in life sciences, material science, and analytical chemistry who are developing next-generation diagnostic assays, studying cellular processes, or characterizing polymer and nanoparticle systems.

Application

• Lipid Droplet Staining and Quantification: A superior fluorescent probe for visualizing and analyzing neutral lipid content in live and fixed cells.
• Biomolecular Interaction Studies: Used in fluorescence spectroscopy and microscopy to investigate protein-lipid interactions and membrane dynamics.
• Polymer and Material Science: Acts as a polarity-sensitive dye for characterizing the hydrophobicity and microenvironments within polymers, micelles, and nanoparticles.
• Diagnostic Assay Development: Serves as a key component in the development of fluorescent sensors and kits for clinical and research diagnostics.
• Environmental Monitoring: Potential use in detecting and analyzing hydrophobic organic pollutants in environmental samples.
• High-Throughput Screening (HTS): Employed in automated screening platforms for drug discovery targeting metabolic disorders like obesity and fatty liver disease.

Basic Information

Product Name	2-Hydroxy Nile Red
CAS No.	188712-75-6
Molecular Formula	C20H17NO3
Molecular Weight	319.36 g/mol
Synonyms	9-(Diethylamino)-5H-benzo[a]phenoxazin-5-one, 2-hydroxy-; 2-Hydroxy Nile Red; Nile Red, 2-hydroxy-; 2-Hydroxy-5H-benzo[a]phenoxazin-5-one, 9-(diethylamino)-; Hydroxynile red; Lipid stain 2-OH derivative; Solvatochromic dye 2-OH NR.
EINECS	Contact for details

Quality Control

Every batch of 2-Hydroxy Nile Red is manufactured and tested under a strict quality management system. We provide comprehensive analytical data to ensure the product meets the high-purity standards required for sensitive research and industrial applications. A detailed Certificate of Analysis (COA), including HPLC purity, spectral data (NMR, MS), and functional performance testing, is supplied with each shipment to guarantee traceability and consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is light-sensitive and should be handled under appropriate conditions to prevent photodegradation. For long-term storage, consider storing desiccated at -20°C.

Specification

Item	Specification
Appearance	Dark red to purple crystalline powder
Identification (HPLC)	Conforms
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Fluorescence Properties	Emission max: 580-620 nm (in ethanol) Excitation max: 530-560 nm (in ethanol)
Solubility	Soluble in DMSO, DMF, ethanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.