Description

Cy3.5(Di Et) CAS NO 186818-80-4 is a cyanine dye derivative, specifically a carbocyanine dye, engineered for advanced fluorescence labeling and detection. This compound is critical for researchers and developers requiring high-performance, near-infrared fluorescent probes with excellent photostability and water solubility. It is primarily needed by professionals in the life sciences, diagnostics, and materials science sectors for applications demanding precise and sensitive molecular imaging.

Application

• Fluorescent Labeling of Biomolecules: Conjugation to antibodies, proteins, peptides, and nucleic acids (DNA, RNA) for detection and quantification in assays.
• Bioimaging and Cell Analysis: Used in fluorescence microscopy, flow cytometry, and in vivo imaging for tracking cellular processes and localization studies.
• Diagnostic Assay Development: Serves as a key component in the development of immunoassays (e.g., ELISA), fluorescence in situ hybridization (FISH), and other clinical diagnostic tools.
• Materials Science Research: Employed in the development of fluorescent sensors, optoelectronic materials, and as a tracer in polymer and nanoparticle studies.
• Pharmacokinetics and Drug Delivery: Used to label and track the distribution, metabolism, and targeting efficiency of therapeutic agents in preclinical research.

Basic Information

Product Name	Cy3.5(Di Et) CAS NO 186818-80-4
CAS No.	186818-80-4
Molecular Formula	C37H45N2O6S2+ • X-
Molecular Weight	~677.9 g/mol (for cation)
Synonyms	Cy3.5 Diethyl; Cyanine3.5 Diethyl; 2-[7-(1,3-Dihydro-1,1-dimethyl-3-(4-sulfobutyl)-2H-benz[e]indol-2-ylidene)-1,3,5-heptatrienyl]-1,1-dimethyl-3-(4-sulfobutyl)-1H-benz[e]indolium hydroxide, inner salt, diethyl derivative; Sulfo-Cyanine3.5 Diethyl; Cy3.5 NHS ester (common reactive form); Near-IR fluorescent dye
EINECS	Contact for details

Quality Control

Our Cy3.5(Di Et) is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and spectrophotometry to verify key optical properties. Certificates of Analysis (COA) detailing batch-specific results are available upon request to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. For long-term storage, desiccate and keep frozen. Allow the product to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Dark green to purple solid or lyophilized powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Maximum (λmax)	~588 nm
Emission Maximum	~604 nm
Extinction Coefficient (ε)	≥ 150,000 cm-1M-1
Solubility	Soluble in water, DMSO, DMF
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.