Description

5-Carboxyrhodamine-6G is a chemically reactive derivative of the classic Rhodamine 6G fluorophore, featuring a carboxylic acid functional group that enables facile covalent conjugation to biomolecules. This compound matters for its exceptional brightness and photostability, making it a superior choice for creating highly sensitive fluorescent probes and labels. It is primarily needed by researchers and manufacturers in the life sciences for advanced applications in fluorescence microscopy, flow cytometry, and diagnostic assay development.

Application

• Fluorescent Labeling Agent: Covalent conjugation to antibodies, proteins, peptides, and oligonucleotides for bioimaging and detection.
• Bioconjugation Chemistry: Serves as a key intermediate in the synthesis of custom fluorescent dyes and probes via amide bond formation.
• Advanced Microscopy: Used in super-resolution microscopy (e.g., STORM, PALM) and confocal microscopy due to its excellent photophysical properties.
• Flow Cytometry: Labeling for cell surface markers and intracellular targets in multicolor flow cytometry panels.
• Diagnostic Assays: Development of fluorescent immunoassays (FLISA) and in-situ hybridization (FISH) kits for clinical and research diagnostics.
• Sensor Development: Integration into chemical sensors and biosensors for environmental monitoring or point-of-care testing.
• Material Science: Incorporation into polymers and nanoparticles to create functional fluorescent materials.

Basic Information

Product Name	5-Carboxyrhodamine-6G
CAS No.	180144-69-8
Molecular Formula	C29H29N2O5
Molecular Weight	485.56 g/mol
Synonyms	6-Carboxyrhodamine 6G; Rhodamine 6G carboxylic acid; 5(6)-Carboxy-X-rhodamine; 6-Carboxy-R6G; 3',6'-Bis(diethylamino)-2-(2-carboxyphenyl)spiro[isoindole-1,9'-xanthen]-3-one; 5-CR 6G; 6-TAMRA precursor; Reactive Rhodamine 6G derivative
EINECS	Contact for details

Quality Control

Our 5-Carboxyrhodamine-6G is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or light will degrade performance. For long-term storage, consider desiccation and storage under inert atmosphere.

Specification

Item	Specification
Appearance	Dark red to purple powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption (λmax)	525 - 535 nm
Emission (λmax)	550 - 560 nm
Extinction Coefficient (ε)	≥ 90,000 cm⁻¹M⁻¹
Fluorescence Quantum Yield	≥ 0.90
Loss on Drying	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.