Description

Dy-560 NHS Ester is a reactive fluorescent dye derivative designed for the efficient labeling of biomolecules, particularly proteins and antibodies, via primary amine groups. This compound is critical for researchers and developers requiring high-performance, stable conjugates for advanced detection and imaging applications. It is essential for professionals in bioconjugation, flow cytometry, fluorescence microscopy, and in vitro diagnostics (IVD) who demand consistent quality and reliable performance in their assays.

Application

• Antibody and Protein Labeling: Conjugation to primary amines (-NH2) for creating fluorescent probes used in immunoassays (ELISA, Western Blot) and immunohistochemistry (IHC).
• Flow Cytometry: Preparation of fluorescently-tagged antibodies for cell surface marker detection and intracellular staining.
• Fluorescence Microscopy & Imaging: Synthesis of targeted imaging agents for cellular and tissue visualization in research and diagnostics.
• Nucleic Acid Labeling: Modification of amine-modified oligonucleotides for fluorescence in situ hybridization (FISH) and other genomic applications.
• Biomaterial and Polymer Functionalization: Incorporation of fluorescent markers into nanoparticles, hydrogels, and other polymeric materials for tracking and analysis.
• Diagnostic Kit Manufacturing: Production of key components for lateral flow assays, microarrays, and other clinical diagnostic devices.

Basic Information

Product Name	Dy-560 NHS Ester
CAS No.	178623-13-7
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	DyLight 560 NHS Ester; Sulfo-Cyanine5 NHS Ester (related dye class); Dy-560 Succinimidyl Ester; NHS ester of Dy-560 carboxy dye; Reactive Dy-560 dye; 560 nm reactive fluorescent label; Amine-reactive Dy-560 fluorophore
EINECS	Contact for details

Quality Control

Our Dy-560 NHS Ester is manufactured under strict quality systems to ensure batch-to-batch consistency and performance. Each lot undergoes rigorous analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency verification, and functional testing in standard conjugation protocols. Certificates of Analysis (COA) detailing all specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark blue or purple solid/powder
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption Max (λmax)	~560 nm
Emission Max (λem)	~580 nm
Extinction Coefficient (ε)	Contact for details
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.