Description

Hoechst 33342 Analog CAS NO 178481-68-0 is a high-purity, cell-permeable fluorescent dye analog used for vital staining of DNA in live and fixed cells. This compound matters for researchers and manufacturers requiring a reliable, high-quality reagent for advanced cellular analysis and diagnostic applications. It is essential for professionals in biotechnology, pharmaceutical R&D, and academic institutions focused on cell biology, cytometry, and high-content screening.

Application

• Live-Cell DNA Staining: For real-time visualization and quantification of nuclear DNA content in viable cells without significant cytotoxicity.
• Flow Cytometry (FACS): As a fluorescent probe for cell cycle analysis and ploidy determination.
• Fluorescence Microscopy: For high-resolution imaging of chromatin and nuclear morphology in fixed or live specimens.
• High-Content Screening (HCS): Utilized in automated imaging platforms for drug discovery and toxicology studies.
• Apoptosis & Cell Viability Assays: To distinguish between live, apoptotic, and dead cell populations based on differential staining.
• Stem Cell Research: For labeling and tracking nuclei in complex co-cultures and 3D organoids.

Basic Information

Product Name	Hoechst 33342 Analog
CAS No.	178481-68-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Hoechst 33342 trihydrochloride analog; Bisbenzimide H 33342 analog; 2'-(4-Ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5'-bi-1H-benzimidazole trihydrochloride; HOE 33342 analog; Nuclear stain 33342 analog; H33342 analog; Vital DNA dye analog; Cell-permeable benzimide dye
EINECS	Contact for details

Quality Control

Our Hoechst 33342 Analog is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science research. Certificates of Analysis (COA) with detailed chromatographic data are available upon request to support your quality assurance and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Fluorescence Performance	Conforms to reference standard
Solubility	Soluble in DMSO, water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.