Description

Tetramethylrhodamine-6-Maleimide is a high-purity, reactive fluorescent dye derivative specifically engineered for selective labeling of thiol groups. This compound matters for researchers and developers requiring precise, covalent attachment of a bright, photostable red-orange fluorophore to proteins, peptides, and other biomolecules. It is primarily needed by professionals in bioconjugation, proteomics, and bioassay development across the pharmaceutical, diagnostic, and life science research sectors.

Application

• Protein Labeling & Conjugation: Covalent attachment to cysteine residues for fluorescence-based studies of protein structure, function, and interactions.
• Biomarker Detection & Imaging: Development of fluorescent probes and antibodies for cellular imaging, flow cytometry, and immunohistochemistry.
• Peptide Tagging: Site-specific modification of synthetic peptides for tracking, purification, or activity studies.
• Diagnostic Assay Development: Creation of fluorescent detection systems for ELISA, lateral flow assays, and other diagnostic platforms.
• Drug Discovery & Screening: Labeling of target molecules in high-throughput screening (HTS) and fluorescence polarization assays.
• Biomaterial Functionalization: Modification of surfaces and nanomaterials with fluorescent tags for biosensing and tracking applications.

Basic Information

Product Name	Tetramethylrhodamine-6-Maleimide
CAS No.	174568-68-4
Molecular Formula	C30H27N3O6
Molecular Weight	525.56 g/mol
Synonyms	TAMRA-6-Maleimide; 5(6)-TAMRA Maleimide; Tetramethylrhodamine-5(6)-Maleimide; 6-TMR Maleimide; 5-(and-6)-Carboxytetramethylrhodamine N-succinimidyl ester maleimide derivative; TMR Maleimide; Rhodamine 6G Maleimide; N-(Tetramethylrhodamine-6-yl)maleimide
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine-6-Maleimide is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency, and identity confirmation. Certificates of Analysis (COA) detailing all specifications and test results are provided with every shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant for long-term stability. For short-term use, the product may be stored at 2-8°C. Allow the vial to equilibrate to room temperature in a dry environment before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	Red to purple solid or powder
Identification (HPLC/UV-Vis)	Conforms
Purity (HPLC)	≥ 95.0%
Extinction Coefficient (ε)	~95,000 cm⁻¹M⁻¹ at 555 nm
Emission Maximum	~580 nm
Solubility	Soluble in DMSO, DMF, acetonitrile
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.