Description

Tetramethylrhodamine-5-Maleimide is a high-purity, reactive fluorescent dye derivative widely used for site-specific labeling of biomolecules. Its core value lies in its maleimide functional group, which enables stable, covalent conjugation to thiol (-SH) groups under mild conditions, making it indispensable for advanced bioconjugation and assay development. This product is essential for researchers and manufacturers in the fields of biopharmaceuticals, diagnostic assay development, and proteomics who require precise and stable fluorescent tagging for proteins, antibodies, and peptides.

Application

• Site-Specific Protein & Antibody Labeling: Covalent conjugation to cysteine residues for fluorescence-activated cell sorting (FACS), immunofluorescence (IF), and microscopy.
• Diagnostic Assay Development: Used as a critical fluorescent probe in lateral flow assays (LFAs), ELISA, and other immunoassays.
• Bioconjugation for ADC Development: A key reagent for creating fluorescently labeled antibody-drug conjugate (ADC) intermediates and linkers for tracking and analysis.
• Proteomics & Biomolecular Imaging: Labeling of peptides and proteins for gel electrophoresis, Western blotting, and in-gel fluorescence detection.
• Nucleic Acid Probe Labeling: Conjugation to thiol-modified oligonucleotides for fluorescence in situ hybridization (FISH) and real-time PCR applications.
• Sensor & Material Science: Functionalization of nanoparticles, polymers, and surfaces for creating fluorescent biosensors and smart materials.

Basic Information

Product Name	Tetramethylrhodamine-5-Maleimide
CAS No.	174568-67-3
Molecular Formula	C26H21N3O5
Molecular Weight	455.47 g/mol
Synonyms	5-TMR maleimide; TMR maleimide; Tetramethylrhodamine maleimide; 5-(and-6)-Tetramethylrhodamine N-(1-maleimidyl); 5-TAMRA maleimide; Rhodamine 6G maleimide derivative; 6-TMR maleimide; 5-Carboxytetramethylrhodamine N-succinimidyl ester maleimide derivative
EINECS	Contact for details

Quality Control

Our Tetramethylrhodamine-5-Maleimide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive life science applications. A comprehensive Certificate of Analysis (COA) detailing purity, absorbance/fluorescence properties, and performance data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Area %)
Absorption Maximum (λmax)	~542 nm (in methanol)
Emission Maximum (λem)	~567 nm (in methanol)
Extinction Coefficient (ε)	~90,000 cm-1M-1
Water Content (KF)	≤ 1.0 %
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.