Description

Sybr Green Ii is a high-sensitivity, asymmetrical cyanine dye widely used as a fluorescent nucleic acid stain. This compound is critical for applications requiring exceptional detection sensitivity and low background fluorescence, offering superior performance over its predecessor. It is an essential reagent for molecular biology, genetic research, and diagnostic assay development. Researchers and industrial laboratories rely on its consistent quality for accurate and reliable results in quantitative analysis.

Application

• Real-Time Quantitative PCR (qPCR): Primary use as a dsDNA-binding dye for monitoring amplification in real-time PCR systems.
• Nucleic Acid Gel Staining: Visualizing DNA fragments in agarose or polyacrylamide gels with high sensitivity, often as a safer alternative to ethidium bromide.
• High-Throughput Screening (HTS): Enabling fluorescence-based assays in drug discovery and genomic research platforms.
• Microarray Analysis: Used for labeling and detection of nucleic acid targets on microarray chips.
• Cell Viability/Cytotoxicity Assays: Application in assays where DNA content is measured as an indicator of cell number or health.
• Next-Generation Sequencing (NGS): Potential use in library quantification and quality control steps.

Basic Information

Product Name	Sybr Green Ii
CAS No.	172827-25-7
Molecular Formula	C32H38N4S2
Molecular Weight	542.80 g/mol
Synonyms	SYBR Green II; SYBR Green II dye; SYBR Green II nucleic acid gel stain; SYBR® Green II; 2-[2-[4-[2-(1,3-Dihydro-1,3,3-trimethyl-2H-indol-2-ylidene)ethylidene]-3(4H)-thiazolylidene]ethylidene]-1,3,3-trimethylindolium; Thiazole orange homodimer; TO-PRO-3 analogue; Asymmetrical cyanine dye
EINECS	Contact for details

Quality Control

Our Sybr Green Ii is manufactured under strict quality systems to ensure batch-to-batch consistency suitable for sensitive research and diagnostic applications. Each lot is characterized for critical parameters including fluorescence properties, purity, and absence of nuclease activity. A comprehensive Certificate of Analysis (COA) detailing spectral characteristics, functional performance in qPCR, and purity assay results is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store the product in its original packaging at 2-8°C. For long-term stability, keep the container in a dry, cool place and avoid exposure to strong alkali or organic solvents. Allow the product to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	Dark green to blue-green solid or solution
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	~497 nm (in aqueous buffer)
Emission Max (λem)	~520 nm (when bound to dsDNA)
Fluorescence Enhancement	> 1000-fold upon binding to dsDNA
Water Content (KF)	< 5.0%
Functional Test (qPCR)	Pass (Efficiency > 90%, Linear R² > 0.99)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.