Description

Sbfi, Tetraamonium Salt, Cell Impermeant CAS NO 172651-12-6 is a specialized, cell-impermeant salt form of the sodium-binding benzofuran isophthalate (SBFI) fluorophore. This compound is critically important as a selective and sensitive intracellular sodium ion (Na⁺) indicator for fluorescence microscopy and imaging applications. It is primarily utilized by researchers and scientists in the fields of cellular physiology, neurobiology, and pharmacology to study sodium ion dynamics and homeostasis in live cells.

Application

• Intracellular Sodium Ion Imaging: As a cell-impermeant probe for loading into cells via patch-clamp pipettes or other microinjection techniques to monitor real-time changes in cytosolic Na⁺ concentrations.
• Neuroscience Research: Investigation of sodium flux in neurons and glial cells associated with action potentials, synaptic transmission, and pathological states.
• Cardiac Physiology Studies: Measurement of sodium currents and homeostasis in cardiomyocytes, relevant to heart function and disease models.
• Cell Signaling Pathways: Probing the role of sodium ions as secondary messengers in various signal transduction cascades.
• High-Throughput Screening (HTS): Potential use in assay development for drug discovery targeting sodium channels and transporters.
• Educational & Core Facilities: A key reagent for academic teaching labs and core imaging facilities specializing in live-cell analysis.

Basic Information

Product Name	Sbfi, Tetraamonium Salt, Cell Impermeant
CAS No.	172651-12-6
Molecular Formula	C₂₆H₂₈N₄O₉ • (NH₄)₄ (or related salt form)
Molecular Weight	Contact for details
Synonyms	SBFI tetraammonium salt; Sodium-binding benzofuran isophthalate tetraammonium salt; 1,3-Benzenedicarboxylic acid, 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-, tetraammonium salt; Cell-impermeant SBFI; SBFI (Na⁺ indicator), salt form; Intracellular sodium indicator SBFI; Fluorescent sodium ion probe.
EINECS	Contact for details

Quality Control

Our Sbfi, Tetraamonium Salt is produced under strict quality protocols to ensure high purity and batch-to-batch consistency, which is critical for reproducible research data. Each lot undergoes comprehensive analytical testing, including HPLC for purity and spectroscopic analysis for functional validation. A detailed Certificate of Analysis (COA) documenting identity, purity, and performance characteristics is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry atmosphere before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid or powder
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95% (Analytical Grade)
Water Content (KF)	≤ 5.0 %
Fluorescence Properties	Ex/Em ~340-360nm / ~500-520nm (Na⁺ bound); Validated performance
Cell Impermeability	Validated (by standard loading test)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.