Description

C6Nbd-Sphingosine,β-D-Galactosyl CAS NO 170212-26-7 is a fluorescently labeled glycosphingolipid derivative, specifically a β-D-galactosylceramide analog tagged with a C6-NBD (7-nitrobenz-2-oxa-1,3-diazol-4-yl) fluorophore on the sphingosine chain. This compound is a critical tool for studying lipid metabolism, membrane trafficking, and glycosphingolipid biology in live-cell imaging and biochemical assays. It is primarily utilized by researchers and developers in pharmaceutical R&D, academic biochemistry, and diagnostic reagent manufacturing who require high-purity, well-characterized probes for advanced lipidomics and cell biology studies.

Application

• Live-Cell Fluorescence Microscopy: As a fluorescent tracer to visualize the intracellular trafficking, localization, and metabolism of glycosphingolipids in real-time.
• Lipid Raft and Membrane Dynamics Research: To investigate the role of glycosphingolipids in membrane microdomain (lipid raft) formation and function.
• Enzyme Activity Assays: Serving as a substrate for studying the activity of glycosidases, glycosyltransferases, and other enzymes involved in sphingolipid pathways.
• Model Compound for Lysosomal Storage Disorders: Used in research related to diseases like Gaucher's or Krabbe disease to understand glycosphingolipid accumulation and therapeutic interventions.
• Drug Discovery Screening: Employed in high-throughput screening platforms to identify modulators of sphingolipid metabolism or glycosphingolipid-related targets.
• Lipidomics Standard: Acts as an internal standard or reference compound in mass spectrometry-based lipidomic analyses for accurate identification and quantification.

Basic Information

Item	Details
Product Name	C6Nbd-Sphingosine,β-D-Galactosyl
CAS No.	170212-26-7
Molecular Formula	C35H55N3O9
Molecular Weight	661.84 g/mol
Synonyms	NBD C6-Galactosylceramide; 6-((N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)amino)hexanoyl)-sphingosine 1-β-D-galactoside; β-D-Galactosyl-N-6-((7-nitro-2,1,3-benzoxadiazol-4-yl)amino)hexanoylsphingosine; C6-NBD GalCer; Fluorescent Galactocerebroside; NBD C6-GalCer; Galactosylceramide, NBD C6-labeled; 1-O-(β-D-Galactopyranosyl)-N-(6-((7-nitro-2,1,3-benzoxadiazol-4-yl)amino)hexanoyl)-D-erythro-sphingosine
EINECS	Contact for details

Quality Control

Our C6Nbd-Sphingosine,β-D-Galactosyl is produced and controlled under stringent quality management systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical characterization, including HPLC for purity, mass spectrometry (MS) for identity confirmation, and NMR for structural verification. Certificates of Analysis (COA) detailing all test results are provided and can be tailored to meet specific project or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below. Allow the product to equilibrate to room temperature before opening to minimize moisture condensation. The material is light-sensitive; prolonged exposure to light should be avoided during handling.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (HPLC-MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Solubility	Soluble in chloroform, methanol, DMSO
Fluorescence Properties	Excitation ~465 nm, Emission ~535 nm (in methanol)
Water Content (KF)	≤ 2.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.