Description

Calcein Blue, Am is a high-purity, cell-permeant fluorescent dye and calcium indicator widely used in biological research. Its core value lies in its ability to form a stable, non-fluorescent complex with intracellular calcium, which becomes intensely fluorescent upon calcium binding, enabling real-time visualization and quantification of calcium ion (Ca²⁺) dynamics. This compound is essential for researchers and professionals in cell biology, neuroscience, and pharmacology for applications such as live-cell imaging, high-throughput screening, and the study of cellular signaling pathways.

Application

• Live-Cell Calcium Imaging: For real-time visualization and measurement of intracellular calcium ion flux in neurons, cardiomyocytes, and other cell types.
• High-Throughput Screening (HTS): Used in automated assays to identify modulators of calcium signaling pathways for drug discovery.
• Fluorescent Microscopy & Flow Cytometry: As a vital stain for detecting and analyzing calcium-dependent cellular processes.
• Neuroscience Research: To study calcium transients associated with neurotransmitter release, synaptic plasticity, and neuronal activity.
• Cell Viability & Apoptosis Assays: Employed in protocols to assess cell health and programmed cell death mechanisms.
• Intracellular Chelation Studies: Useful for investigating calcium buffering and sequestration within cellular compartments.

Basic Information

Product Name	Calcein Blue, Am
CAS No.	168482-84-6
Molecular Formula	C₄₆H₄₄N₂O₂₃
Molecular Weight	980.85 g/mol
Synonyms	Calcein Blue AM; Calcein Blue, acetoxymethyl ester; 2,2'-[2-[Bis(carboxymethyl)amino]-5-methylphenoxy]methyl-6-hydroxy-4-methyl-8-oxo-4H,8H-pyrano[3,2-g]chromene-3,5,7-tricarboxylic acid, 3,5,7-tris(acetoxymethyl) ester; 4-Methylumbelliferyl-EDTA; Fluorescent Calcium Indicator Calcein Blue AM; CB-AM
EINECS	Contact for details

Quality Control

Our Calcein Blue, Am is manufactured under strict quality systems to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectral analysis for identity confirmation, and functional validation in cell-based assays. A detailed Certificate of Analysis (COA) providing purity, absorbance/fluorescence profiles, and performance data is supplied with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange solid
Identification (HPLC/UV-Vis)	Conforms to reference standard
Purity (HPLC)	≥ 95.0%
Absorption Max (λmax)	325 - 335 nm
Emission Max (λem)	435 - 445 nm
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF
Functional Activity	Validated in cell-based calcium assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.