Description

4,5-Benzo-1'-Ethyl-3,3,3',3'-Tetramethyl-1-(4-Sulfobutyl)Indodicarbocyanin-5'-Acetic Acid n-Succinimidyl Ester is a reactive, near-infrared (NIR) fluorescent dye derivative designed for high-sensitivity labeling applications. This compound matters for its ability to form stable amide bonds with primary amines, enabling the covalent tagging of proteins, peptides, antibodies, and other biomolecules for detection and analysis. It is primarily needed by researchers and manufacturers in the biotechnology, pharmaceutical, and diagnostic sectors for applications such as fluorescence microscopy, flow cytometry, and in vivo imaging.

Application

• Bioconjugation and Probe Development: Covalent labeling of antibodies, proteins, and oligonucleotides for creating fluorescent probes and detection reagents.
• Bioimaging and Microscopy: Used in near-infrared fluorescence microscopy (NIRF) and in vivo imaging studies due to its favorable excitation and emission profiles in the NIR window.
• Flow Cytometry: Conjugation to cell surface markers or intracellular targets for high-content screening and cell analysis.
• Diagnostic Assay Development: Key component in the manufacture of fluorescent immunoassays (FLIA) and other clinical diagnostic kits.
• Protein and Biomolecule Tracking: Studying protein-protein interactions, localization, and turnover in complex biological systems.
• Material Science: Functionalization of nanoparticles and polymers for advanced material applications and sensor development.

Basic Information

Product Name	4,5-Benzo-1'-Ethyl-3,3,3',3'-Tetramethyl-1-(4-Sulfobutyl)Indodicarbocyanin-5'-Acetic Acid n-Succinimidyl Ester
CAS No.	167638-53-1
Molecular Formula	C41H45N3O7S
Molecular Weight	723.88 g/mol
Synonyms	IR-783 NHS Ester; Cy7.5 NHS Ester; Sulfo-Cy7.5 NHS Ester; 1-(4-Sulfobutyl)-3,3,3',3'-tetramethyl-4,5-benzo-1'-ethylindodicarbocyanin-5'-acetic acid N-succinimidyl ester; NIR-783 NHS ester; IRDye® 783 NHS Ester; Seta™ 750 NHS Ester; Seta-750 Succinimidyl Ester
EINECS	Contact for details

Quality Control

Our production of this high-value biochemical adheres to stringent quality protocols. Each batch is characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry to confirm identity, purity, and reactivity. Certificates of Analysis (COA) detailing purity, absorbance/emission maxima, and functional performance are provided with every shipment to ensure consistency for your critical research and development work.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to warm to room temperature in a desiccator before opening to prevent condensation and hydrolysis of the active NHS ester group.

Specification

Item	Specification
Appearance	Dark green to blue solid
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Absorption Maximum (λmax)	783 ± 5 nm
Emission Maximum (λem)	810 ± 10 nm
Extinction Coefficient (ε)	≥ 200,000 M-1cm-1
Water Content (KF)	≤ 1.0%
Functional Activity (Labeling)	Conforms

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.