Description

5-[Bromoacetamido]Tetramethylrhodamine is a reactive, fluorescent labeling reagent widely used for the covalent modification of biomolecules. This compound is valued for its ability to introduce a bright, photostable rhodamine fluorophore onto target molecules, enabling sensitive detection and imaging. It is an essential tool for researchers and manufacturers in the fields of bioconjugation, flow cytometry, fluorescence microscopy, and diagnostic assay development.

Application

• Bioconjugation and Probe Synthesis: Covalent labeling of antibodies, proteins, peptides, and oligonucleotides for fluorescence-based applications.
• Flow Cytometry: Preparation of fluorescently-tagged antibodies and other ligands for cell surface marker analysis and immunophenotyping.
• Fluorescence Microscopy (e.g., Confocal, Super-Resolution): Creating high-performance imaging probes for subcellular localization and tracking studies.
• Diagnostic Assay Development: Used in the manufacture of reagents for ELISA, lateral flow assays, and other in vitro diagnostic (IVD) platforms.
• Biomolecular Interaction Studies: Labeling of ligands for fluorescence polarization (FP) or fluorescence resonance energy transfer (FRET) assays.
• Chemical Biology Research: A versatile tool for studying protein function, localization, and dynamics in live or fixed cells.

Basic Information

Item	Detail
Product Name	5-[Bromoacetamido]Tetramethylrhodamine
CAS No.	166442-38-2
Molecular Formula	C29H28BrN3O5
Molecular Weight	578.46 g/mol
Synonyms	5-TAMRA-Br; 5-Carboxytetramethylrhodamine Bromoacetamide; 5-TAMRA Bromoacetamide; 6-Bromoacetamido Tetramethylrhodamine; TAMRA-5-Bromoacetamide; 5-(Bromoacetamido)tetramethylrhodamine; Bromoacetyl TAMRA; Reactive TAMRA dye
EINECS	Contact for details

Quality Control

Our 5-[Bromoacetamido]Tetramethylrhodamine is produced under strict quality management systems to ensure batch-to-batch consistency and high performance. Each lot undergoes comprehensive analytical testing, including HPLC for purity, spectrophotometry for labeling efficiency verification, and identity confirmation. Certificates of Analysis (COA) with detailed specifications are provided to support your research and manufacturing quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the vial to equilibrate to room temperature in a dry environment before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	Dark red to purple solid
Identification (HPLC/MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Absorption (λmax)	~546 nm
Emission (λmax)	~576 nm
Extinction Coefficient (ε)	~90,000 cm-1M-1
Solubility	Soluble in DMF, DMSO, acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.